Obtained slices were incubated for 2 hours at 60AdegC and administered with two different xylol
for clarification and rehydrated with decreasing alcohol series.
The slides were passed through a series of graded alcohol and xylol
, then stained with hematoxylin and eosin (H&E).
The alcohol-preserved specimens were dehydrated following the usual alcohol series (70-100%) and cleared in xylol
before being included in paraplax and paraffin.
Tissue samples from livers and small intestines of the fish were fixed in 10% neutral formaldehyde for 48 h before transferring to a graded alcohol (70%, 80% and 96%) series, made transparent in xylol
and embedding in paraffin.
(2008), where the high hydrofibicity is between 66.67 to 100%, average between 33.37 to 66.66%, and low hydrophobicity is 0 to 33.33%, we see that the adhesion of LA1 cells to xylol
is in the range of medium adhesion, whereas for the ethyl acetate and chloroform solvent the adhesion may be considered high.
The specimens were dehydrated in different grades of alcohol, cleared in xylol
, embedded in paraffin wax, sectioned at 4-6 um thick, and stained with Hematoxylin and Eosin.
The sections were then deparaffinized in xylol
and rehydrated in successive alcohol baths.
After that, samples were dehydrated by submerging into 100% ethanol 3 times for 30 minutes each, cleared in xylol
3 times for 15 minutes each, and embedded in liquid paraffin at 60[degrees]C 3 times for 30 minutes each, and finally included in a solid paraffin block.
The slices were deparaffinized in a stove at 45[degrees]C for 30 minutes and, afterwards, placed in xylol
for 5 minutes.
The nerve specimens, after routine tissue processing, were cut into 3-[micro]m-thick transverse, oblique, and longitudinal serial sections and deparaffinized in xylol
for 10 minutes.
After six ethanol (from 70%-100%) and xylol
baths, they were embedded in paraffin.
Skin samples from both the wound and comparable contralateral normal skin were fixed in 10% neutral-buffered formalin, dehydrated in graded ethanol, and cleared in xylol
. The specimens were then embedded in paraffin, and sections of 5 mm in thickness were stained, using hematoxylin and eosin (H&E) and Masson green trichrome, and studied by a routine light microscope (Olympus, Tokyo, Japan).
After one-night incubation at 37-40[degrees]C, dewaxing of the 4 [micro]m sections was completed by another 45 minutes of incubation at 65[degrees]C and immersion in xylol
for 20 minutes.
Representative tissue pieces were also collected from the tumor mass and fixed in 10 percent buffered formalin, processed through alcohol and xylol
and embedded in paraffin.
Deparaffinize slides in Xylol
solution 2x, every 15 minutes.