Subcultures of these organisms isolated from human infections were made and the stock cultures
maintained in the laboratory.
However, establishing some stock cultures
in MSM medium in advance of the students' starting date is helpful in avoiding a delay in population growth that often occurs if Euglena are transferred from the pea medium into the MSM medium.
The facility maintains stock cultures
of four phage types of SE (8, 14B, 23, and 24) for vaccine production.
RFLP analysis of ssrDNA of the stock cultures
both before and after the experiments verified that each stock culture
contained a single algal clade.
Test cultures were inoculated from the stock culture
of each species at the curve of optimum population growth.
Ten percent (V/V) of each stock culture
was inoculated to 20 L of medium cultivated at room temperature with continuous illumination and aeration by bubbling air-line in plastic carboy tank.
Additionally, the choice of container type, differed depending on size, is an operational practice for containerized tree stock culture
(Faure- Lacroix et al.
Frey's medium (180 l) was added in 1st well to 12th well followed by adding stock culture
of crude extract (10 L) of each plant in 1st well.
Laboratory stock culture
of Staphylococcus epidermidis ATCC 12228 (mannitol salt agar test)
Prevalence of Candida dubliniensis isolates in a yeast stock culture
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Working stock culture
was obtained by subculturing mycelia from covered slants to screw-cap vials (16 by 125 mm), each vial containing a 10-ml PDA slant.
Mycoplasma activated stock culture
was centrifuged at 10,000 g for 30 min, washed once in normal saline (pH 7.
This is done by using a transfer loop to move bacteria from the stock culture
to the tube.
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