targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants.
The restriction site for NdeI enzyme was added before the start codon
of confirmed gene sequence and complete open reading frame was ligated into pET22b (+) expression vector using NdeI and EcoRI restriction sites for both the gene and the vector.
Translation proceeds sequentially along the mRNA molecule from the start codon
nearest the ribosome-binding site terminating at stop codons and reinitiating at the next start codon
53 1 Polymorphisms in promoter region numbered relative to the translation start site; Adenine of the start codon
ATG is counted as +1 (GenBank accession No.
According to the stop and start codons
, exon can be classified as: (i) a single exon gene which starts with a start codon
and ends with a stop codon, (ii) an initial exon that begins with a start codon
and ends with a donor site, (iii) a terminal exon that starts with an acceptor site and ends with a stop codon, (iv) and an internal exon that starts with an acceptor site and ends with a donor site .
The first exon is untranslated, and the start codon
(AUG) is located at the beginning of exon 2 (1).
This polymorphism, resulting from a T[right arrow]C transition, introduces codon 4 as the start codon
resulting in a shorter isoform of the VDR.
The GSTP1 313A[right arrow]G (rs1695), NQO1 609C[right arrow]T (rs1800566), MBL2 codon 54A[right arrow]G (rs1800450), ABCB1 3435C[right arrow]T (rs1045642), MTHFR 677C[right arrow]T (rs1801133), and VDR start codon
FokI polymorphism (rs2228570) were genotyped by DNAPrint Genomics (Sarasota, FL) in a multiplex PCR followed by single base extension.
5-kb upstream region from the start codon
probably corresponding to the promoter revealed a plant MYB-binding domain (AACCAAAC) at [approximately equal to] 1400 bp upstream and a G-box core sequence (CACGTG) at [approximately equal to] 1250 bp upstream of the start codon
Next, each ORF segment was aligned with the start codon
beginning at the zero location (ORFs indicated as being in the opposite direction were computed as reverse compliments and aligned).
Primers (200ug/ml) were designed flanking the c1 gene and contained a ribosome binding site upstream of the c1 gene start codon
The AUG mRNA codon codes for methionine and also acts as the start codon
for all proteins.
The start site or start codon
is the first codon of the first exon.
Such mRNAs begin with a 5'-AUG-3' start codon
at or very near the 5' terminus of the message, and their lack of 5' untranslated leaders means that they also lack conventional Shine-Dalgarno homologies believed to exist in most prokaryotic messages.
The functional FokI polymorphism is the most important start codon
polymorphism in VDR gene.