The general measurements of the nuclei (longest, smallest and mean axis, volume, area, perimeter and V/A ratio) of cells of the basal and spinous layers of the junctional epithelium showed a statistically significant reduction in the treated group.
The nuclei were smaller in the basal and spinous layers, as observed by the smaller values for longest, smallest and mean diameters, perimeter, area, volume and V/A ratio.
In our patient, histopathology revealed hyperkeratosis, acanthosis, marked vacuolization of granular and upper spinous layers
with coarse keratohyalin granules.
Histologically, the skin lesions showed eosinophilic granular intracytoplasmic inclusion bodies in basal and spinous layers
of epidermis, compatible with poxvirus infection; superficial ulceration and extensive intraepidermal abscesses were also found.