slide by

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This novel method of stain quantification enables several improvements over existing methods including: 1) the detection of tissue on a histological slide by automated robotic microscopes; 2) improvements to auto-focus algorithms ensuring that automated digital imaging of histological slides is confined to tissue areas only; 3) improvements to automated image segmentation and the identification of tissues and their structural features; 4) improvements to the identification of individual stains when multiple stains are used, and 5) improvements to the quantification of the extent of staining.