Estimation of seminiferous tubules
length: The length density of the tubules was calculated using an unbiased counting frame (25) at the final magnification of 148x using the following formula:
Total thirty seminiferous tubules
were observed in each group.
Group 3, Group 4 & Group 5 shows seminiferous tubules
with few spermatozoa and interstitial space with interstitial cells (Leydig cells) shows presence of few sperm cell within the lumen of the seminiferous tubules
vascular interstitial cells due to vascular interstitial cell moving spermatozoa along the lumen of the tubules EGME Treatment (Fig.
In addition to the severe exfoliation of the degenerative and germinative epithelium, other alterations were observed, including a reduction in the number of spermatogonia and spermatocyte layers, accumulation of proteinaceous material in the lumen of the seminiferous tubules
and absence of epithelial cell stereocilia in the epididymis (Figure 2).
Percentage of seminiferous tubules
containing multinucleated giant cells
This study evaluates the reversibility of KISS1 gene vaccine for immunocastration in ram lambs in terms of changes in anti-KISS1 antibody titers, serum testosterone levels, scrotal circumference, testicle weight, and spermatogenesis in the seminiferous tubules
. The safety of immunocastration against KISS1 gene vaccine was evaluated in terms of the integration of fusion gene KS (KISS1- HBsAg-S, HBsAg-S refers to hepatitis B surface antigen S) of KISS1 gene vaccine and genomic DNA of the host cell in vaccinated ram lambs.
Hence, seminiferous tubules
only contain spermatocytes at the end of the development of germinal cells.
Also previous studies have showed that ghrelin-ip cells were distributed in the seminiferous tubules
and interstitial tissues of the testes of human, mouse and sheep (David et al., 2005; Gaytan et al., 2004; Ilhan and Erdost, 2013).
The important characteristic of this stage was spermiation of the elongated spermatids into the lumen of seminiferous tubule
The mean diameter of seminiferous tubules
was 256.13 +- 6.24um; 254.62 +- 4.52 um and 254.11 +- 3.27 um in groups A, B and C respectively.
The hypothesis stated in our study is as follows: Are the seminiferous tubule
structure and Leydig cell number similar in PBS and normal fetuses?
The technique of seminiferous tubule
culture has been described previously (Hue et al., 1998; Staub et al., 2000).
Also this vitamin improves testis weight, seminiferous tubule
diameter and thickness of the germinal epithelium .
 In a study performed by Susheela and Kumar,  it was seen that chronic fluoride toxicity caused regression of seminiferous tubule
and cessation of the spermatogenesis in the rabbit.
Key words: Guinea fowl, histomorphology, seminiferous tubule
's epithelium, serum testosterone, testicular cycle.