Photons are emitted from iodine 125 ([sup.125]I) in a single beam and detected by a scintillation counter. The patient places her forearm or lower leg in a water bath to minimize the interference by soft tissues, especially fat.
Wide-angle X-ray scattering (WAXS) patterns in the range of scattering angles (20) 5-40 deg were measured with a DRON-2, 0 diffractometer (copper radiation, [Lambda] = 0.154 nm; nickel filtering of the reflected beam; step-by-step scanning regime; recording of scattered radiation with a scintillation counter and digital conversion).
The radiolabeled product was extracted with hexane and counted with a liquid scintillation counter. Analysis of some extracts by HPLC (3) showed that the radio-labeled product eluted in a single peak with the same retention time as MF.
Luciferase activity of 25 [micro]L cell lysate was measured with the Luclite Luciferase Reporter gene assay kit (Perkin-Elmer, Brussels, Belgium) according to the manufacturer's instructions using 25 [micro]L Luclite solution on a Topcount liquid scintillation counter (Perkin-Elmer).