immunoelectrophoresis

(redirected from rocket immunoelectrophoresis)
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Words related to immunoelectrophoresis

electrophoresis to separate antigens and antibodies

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Concentration Diameter of ring ([micro]g/ml) (mm) 50 0.5 75 1 100 7 125 12 150 17 Table 3: Rocket immunoelectrophoresis. Antigen concentration Rocket height (in mg/ml) (in mm) 50 1.6 75 3.9 100 5.3 125 7.4 Table 4: Reproducibility of allergen reactions by ELISA.
Rocket immunoelectrophoresis was performed in 1.5 mm thick, 1% agarose (Litex HSA; Litex Industries) in 0.02 mol/L Tris-barbital buffer, pH 8.6, containing monospecific rabbit anti-PINP prepared as described previously (6).
After incubation, the samples were subjected to size chromatography, and the distribution of PINP was analyzed by rocket immunoelectrophoresis, ELISA, and RIA.
In contrast, incubation of fraction 27 (the low-molecular weight form) at 37[degrees]C for 72 h did not produce marked changes in either the absorbance profile at 280 nm or the distribution of PINP as analyzed by rocket immunoelectrophoresis (Fig.