2] modification at A37 indeed attenuated reverse transcription
when primer r2 was used, whereas reverse transcription
was unaffected when primer r1 was used.
Number of newly febrile horses and results of RT-PCR and a virus neutralization test, Japan, 2014 * Sep 1-7, Sep 8-14, Sep 15-21, Sep 22-28, Test n = 3 n = 3 n = 12 n = 15 RT-PCR NT NT NT 4/5 ([dagger]) Neutralization NT NT 4/4 ([double 4/4 dagger]) Sep 29-Oct 5, Oct 6-12, Oct 13-19, Test n = 13 n = 16 n = 7 RT-PCR 11/13 6/16 3/7 Neutralization 5/5 3/6 NT Oct 20-26, Oct 27-Nov Test n = 2 2, n = 6 RT-PCR 1/2 0/6 Neutralization NT NT * NT, not tested; RT-PCR, reverse transcription
stem and loop secondary RNA structure) has a substantial impact on the efficiency of reverse transcription
and the PCR.
5 units of Taq DNA Polymerase, 6 [micro]L of the reverse transcription
reaction described above, and 20 pmol each of CYP1A1 primers.
RNA from 2 ferret HEVs was randomly selected, and the full-length genome was amplified by using reverse transcription
PCR with primers based on nucleotide sequences derived from strains from the Netherlands and United States (Table 1).
Real-time reverse transcription
(RT)-PCR is the most sensitive method to measure changes in mRNA concentrations.
QuantiGene Plex advances state-of-the-art gene expression analysis, by allowing researchers to accurately and precisely quantitate expression of multiple mRNA targets in a single well without the need for RNA purification, reverse transcription
, or target amplification, and to be able to detect percent differences in expression, rather than fold differences measured by current technologies.
Applied Biosystems (NYSE:ABI), an Applera Corporation business, today announced a line of reagents that provide researchers with new solutions for streamlining the process of performing quantitative reverse transcription
PCR in RNA-based research.
Also to provide multiplex real time PCR master mix and multiplex real time one step reverse transcription
master mix for those in-house assays.
Given the likely variation in the preparation of RNA, the quality and formulations of commercial reverse transcription
(RT)-PCR enzymes and reaction mix components and changes in thermal cyclers since 1988, not surprisingly many, perhaps most, of these assays are not being used in the manner they were originally described.
We performed reverse transcription
with the DyNAmo SYBR Green 2-step qRT-PCR Kit (Finnzymes) according to the manufacturer's instructions.
Requiring only seconds of labor per sample, the RNA Catcher provides a high throughput approach for isolation of mRNA samples to be analyzed by reverse transcription
and quantitative PCR (RT-PCR).
The ArrayPlate qNPA technology offers highly accurate, sensitive and reproducible test results without the need for extraction, reverse transcription
Knockdown of all members of the PAF1 family of proteins enhanced HIV-1 reverse transcription
and integration of provirus.
The RNA samples were subjected to 1 cycle of reverse transcription
(42[degrees]C, 10 min) followed by 5 min at 95[degrees]C.