Results of the above studies indicated that both serum and prostatic fluid specimens contained free PSA as well as free ACT.
Because prostatic fluid and seminal plasma are rich in zinc, 390-450 mg/L (6 mmol/L) and 150-200 mg/L (2 mmol/L), respectively , and PSA is known to be inhibited by zinc , zinc could play a role in preventing the formation of the PSA-ACT complex.
Results of the present study demonstrate that, although both serum and prostatic fluid contain PSA and ACT, a significant amount of PSA-ACT complex is detected in serum but not in prostatic fluid.
An alteration in the pattern of migration for ACT from prostatic fluid was associated with the absence of PSA-ACT complex in these specimens.
That PSA and ACT did not form a significant amount of the complex in prostatic fluid may be physiologically important.
The lack of significant amount of complex formation between PSA and ACT in the prostatic fluid was possibly attributable, in part, to the 6 mmol/L concentration of zinc in this fluid .
3B, our detection system was capable of detecting fragmented PSA in prostatic fluid, apparently an intact (30-kDa) form of PSA is present in human serum that is incapable of reacting with ACT.
In summary, results of the present study demonstrate that PSA-ACT complex is readily detected in serum but not in prostatic fluid or in seminal plasma.
Two-dimensional characterization of prostatic acid phosphatase, prostate specific antigen and prostate binding protein in expressed prostatic fluid.