We recently described the structure of a novel metabolite in rat brain after oral administration of salvianolic acid B with chemical name (2E)-2-{6-[(E)-2-carboxylvinyl]-2,3-dihydroxyphenyl}-3-(3,4-dihydroxy-phenyl) propenoic acid (Chinese patent: CN 200710015108.8), designated SMND-309.
1, purity >99.5%), with molecular formula [[C.sub.18][H.sub.14][O.sub.8]] and molecular weight 358.3, soluble in saline water, was obtained from the Department of Chemistry, NDCenter; The (1) H- and (l3) C-NMR spectra were in good agreement with the structure for (2E)-2-{6-[(E)-2-carboxylvinyl]-2,3-dihydroxyphenyl}-3-(3,4-dihydroxyphenyl) propenoic acid. A23187, Malate, glutamate, Succinate, rotenone, dihydrorhodamine, rhodamine, nitrilotriacetic acid, antimycin, hematoxylin and eosin were purchased from Sigma-Aldrich, St.