In the IVF laboratory, pronuclear
status, subsequent development rate and embryo morphology are common parameters in the selection of good quality embryos.
They point to a growing pronuclear
tendency in the environmental movement and see it as a hopeful sign.
Generating transgenic mice using oocyte pronuclear
DNA microinjection includes five basic steps: purification of transgenic construct, harvesting donor zygotes, microinjection of transgenic construct, implantation of microinjected zygotes into pseudo-pregnant recipient mice, and genotyping of transgene expression in founder mice (3).
/n-% Stained cell (total) 181.9[+ or -]91.4 182.5 244.0[+ or -]125.2 Ki-67 stained cell 58.8[+ or -]37.4 51.5 81.0[+ or -]41.4 Ki-67 staining rate 32.6[+ or -]14.5 30.6 36.9[+ or -]18.3 Stained cell (total) 178.2[+ or -]91.2 162.5 218.8[+ or -]116.7 PCNA stained cell 85.9[+ or -]50.7 64.5 123.6[+ or -]90.5 PCNA staining rate 48.3[+ or -]17.0 48.3 58.1[+ or -]23.9 Recurrence(+) Median p Stained cell (total) 224.0 0.091 (m) Ki-67 stained cell 77.0 0.076 (m) Ki-67 staining rate 37.8 0.395 (m) Stained cell (total) 198.0 0.319 (m) PCNA stained cell 83.0 0.096 (m) PCNA staining rate 57.3 0.120 (m) (m) Mann-whitney u test / [chi square] Chi-square test PCNA: pronuclear
cell antigen Table 3.
Cytoplasmic maturation play a key role in male prokaryotic formation (cytoplasmic maturation can be determined by theAdegCcurrence of male pronuclear
formation), meiotic cell cycle development, initiation pathways compulsory for genetic and epigenetic processes and pre-implantation embryo developing capacity (Oehninger, S.
Human Fertilization Status Based on Pronuclear
Origin and/or Centrosome
Liang et al., "Active and passive demethylation of male and female pronuclear
DNA in the mammalian zygote," Cell Stem Cell, vol.
A problem commonly encountered in ICSI technique is the low rate of viability of the microinjected oocytes and inconsistent level of male pronuclear
formation after microinjection.
"It is deeply moving and highly rewarding to see that embryos frozen 24.5 years ago using the old, early cryopreservation techniques of slow freezing on day one of development at the pronuclear
stage can result in 100 percent survival of the embryos with a 100 percent continued proper development to the day-3 embryo stage," said Sommerfelt.
Different processes of pronuclear
events in pressure-treated and CB-treated zygotes at the second meiosis in scallop.
A prolongation was observed in time to pronuclear
fading and t2 cleavage times in time-lapse in the non-smoker group (p3 follicles reached >17mm diameter; human chorionic gonadotropin (hCG) trigger which consisted of subcutaneous (sc) injection of recombinant human chorionic gonadotropin (rhCG) (Ovitrelle, Merck Serono, Turkey) or intramuscular urinary human chorionic gonadotrophin (uhCG) (Pregnyl MSD, Turkey) was administered.
scoring as a predictor of embryo quality in in vitro fertilization program.
In second round SCNT, one hundred and fifty-five pronuclear
stage embryos were transferred into the oviducts of 13 recipient goats.
Greenhill and Friedman write in their 1974 obstetrical textbook, "The term conception refers to the union of the male and female pronuclear
elements of procreation from which a new living being develops ...
Elective cryopreservation of all pronuclear
oocytes after GnRH agonist triggering of final oocyte maturation in patients at risk of developing OHSS: a prospective, observational proof-of-concept study.