The standard curve was completed by serially diluting the virus stock and extracting the RNA from each dilution, according to the previously mentioned RNA extraction protocol, while simultaneously titrating each dilution in a standard plaque assay
aureus and (B) different type plaque assay
showing different type of plaque morphology
A) Plaque assay
results for recombinant SINV-3 (AcSINV-3) transfection of Sf21 cells indicating the dilution used for each plate.
The viral plaque assay
was performed as previously described to determine cardiac viral load.
cholerae strains used as indicators in plaque assays
or host for phage preparations were from either clinical or environmental sources.
A sampling of topics from the first volume: bacteriophage enrichment from water and soil, isolation of phage via induction of lysogens, enumeration of bacteriophages by double agar overlay plaque assay
, basic phage electron microscopy, construction of phage mutants, generalized transduction, and preparation and characterization of anti-phage serum.
For ozone experiments, the virus titers were determined by plaque assay
, and the virus suspension was stored at 4[degree]C within 24 hours.
YFV clinical isolate V-341 (provided by the Laboratorio de Virologia, Instituto Nacional de Salud, Bogota) was amplified on Vero cells and titrated in a plaque assay
Nasal-wash samples obtained from 101 children prospectively enrolled during the RSV season between November 2004 and February 2005 were analyzed for viral pathogens via cell culture, RSV quantification via plaque assay
, and cytokine and chemokine concentrations via the Bio-Plex Suspension Array System.
Aliquots were processed for standard plaque assay
and DNA isolation (Qiagen QIAamp DNA Blood Mini Kit).
In the "proof of concept" clinical trial (also referred to as Small Plaque Assay
or Microplaque Assay), 100 micronL of ATL1101 cream will be applied to areas of patients' skin, once every two days, over a one-month period.
A range of bacteriophage surrogates of mammalian viruses was employed (see Table 1) and efficacy was assessed by comparing the initial and final viral titres on the surfaces using a plaque assay
and replication of Tipula iridescent virus in Spodoptera frugiperda ovarian cells.
Mosquitoes were pooled and then tested for the presence of WNV using vero cell plaque assay
 and a fluorogenic real-time polymerase chain reaction (PCR) assay ([TaqMan.
Subsequently, plaque assay
results revealed distinct plaques 3 days after inoculation within wells inoculated with blood and serum (1).