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Key words: allophycocyanin (APC); equivalent reference fluorophores (ERF); fluorescein isothiocyanate (FITC); fluorescence; multicolor flow cytometer; Pacific Blue (PB); phycoerythrin (PE).
was studied using response surface methodology on the production of phycoerythrin .
Familiarity with two popularly used conjugate dyes in flow cytometry, fluorescein and phycoerythrin, as well as the use of dyes for assessment of cell viability were also suggested.
The following monoclonal antibody panel was used for staining: fluorescein isothiocyanate (FITC)-labelled anti-Lineage (Lin-) panel (CD3/CD14/ CD16/CD19/ CD20/CD56) (BD, USA), peridinin chlorophyll protein (PerCP)-labelled anti-HLA-DR (BD), phycoerythrin (PE)-labeled anti-CD11c (BD PharMingen, USA), and either allophycocyanin (APC)-labelled CD40 (PharMingen, USA), -CD86 (PharMingen, USA), -CXCR4 (BD PharMingen, USA), or -CCR5 (BD PharMingen, USA).
The phycoerythrin meter detects red cyanobacteria fluorescence in all kinds of environments, from mangrove swamps to oligotrophic blue water.
reported to contain a phycoerythrin (PE) pigment (PE-III) as well as the
The color was produced by the natural fluorescence of phycoerythrin, the primary light-harvesting pigment in many cyanobacteria.
When phycoerythrin absorbs the portions of sunlight that penetrate to the corals' depth of at least 45 meters, it releases orange light.
The products of these genes, phycocyanin and phycoerythrin, respectively, accumulate in the light-harvesting complexes of F.
Phycoerythrin (PE)-labeled monoclonal antibody against human glycophorin A (anti-glycophorin A-RPE) was from Dako (Glostrub, Denmark), and fluo-4 acetoxymethyl ester (Fluo-4 AM) was from Molecular Probes (Eugene, OR, USA).
Finally, the beads and the phycoerythrin signal were analyzed in a Bio-Plex 100 Analyzer (Bio-Rad), which gave results in median fluorescent intensity (MFI).
5] cells in a total volume of 50 [micro]L were labeled, with the following mouse antihuman antibodies, which were conjugated with either fluorescein isothiocyanate (FITC) or phycoerythrin (PE): CD34-PE (BD Pharmingen, USA), CD45-FITC (BD Pharmingen), CD73-PE (BD Pharmingen), CD9O-FITC (AbD SeroTec, USA), and CD105-FITC (AbD SeroTec).
Louis, MO, USA) and 10 [micro]L phycoerythrin (PE) labeled mouse IgG anti-CD19 (BD Pharmingen, San Diego, CA, USA ) were added to the antibody-blood mixture.
6] cells/[micro]l) and 10 [micro]l of IgG1- phycoerythrin ([10.
Also study on different pigments such as Chlorophyll, Carotenoid and phycoerythrin content revealed that marine macroalgal species and their concentrations vary with different divisions (Kumar et al, 2009 b).
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- phrenic nerve
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- Phrynosoma cornutum
- phthalic acid
- phthalic anhydride
- Phthirius pubis
- Phthorimaea operculella
- Phycomycetes group
- Phyllitis scolopendrium
- Phyllocladus alpinus
- Phyllocladus asplenifolius
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- Phyllodoce breweri
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- phycocyanobilin:ferredoxin oxidoreductase
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- Phycological Society of America
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