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(1992) reported that the ability of murine macrophages to kill opsonized Escherichia coli was not impaired by 'anaerobiosis' (p[O.sub.2] < 5mmHg).
Adherent hemocytes were washed with 400 [[micro]liter] FSW before being overlaid with 50 [[micro]liter] yeast (Baker's yeast type II, Sigma Chemicals; 5 x [10.sup.6] yeast/ml) that had been prepared and opsonized with S.
The MLI was also evaluated after stimulation by particulate (opsonized zymosan) and soluble (phorbol myristate acetate, PMA) surface stimulating agents ('stimulated MLI').
Macrophages/microglia not only remove pathogenic microorganisms, their toxins, and noxious agents opsonized with antibodies but also send activation signals to other cells of the immune system .
After incubation, serum opsonized zymosan (25 uL) and intracellular reactive oxygen species detecting probe (25 uL), luminol were added into each well, except blank wells containing only HBSS++.
pneumoniae ([10.sup.4] CFU in 100 [micro]L) that was opsonized with mouse serum for 30 min were incubated at 37[degrees]C for 1 h with shaking on a rotator in 5% C[O.sub.2] atmosphere.
At an indicated time the cells were washed twice with PBS and the opsonized yeast suspensions were added at a MOI of 5.
Lactosome on second dosage was opsonized by the anti-Lactosome antibody soon after the administration and entrapped by reticuloendothelial system (RES).
The dominant retention in the liver is caused by the opsonization of the complexes, since negatively charged antibody tends to bind with positively charged complexes in the bloodstream and subsequently, opsonized complexes are recognized by the phagocytic system .
The strains were categorized into three groups-non-opsonized, 1-50 per cent opsonized and 51-100 per cent opsonophagocytosed.
Macrophages were harvested after 5 days and the phagocytic activity was determined using opsonized SRBC (Mehera and Vaidya, 1993) and compared with the macrophages isolated from the untreated vehicle control.
Additionally, to examine in vivo effects of NP, we also analyzed the effects of NP itself and the synergistic effects of NP and a typical inflammatory agent, opsonized zymosan, on human whole blood including neutrophils.
There is activation of complement in the new host, and the promastigotes are opsonized and phagocytized by macrophages.
The data obtained from this nvestigation suggested that the non opsonized particles of ALCAP and TCP materials exhibited an increase in cell number at both the 48 and 72 hour phases.
Yan et al., "Human [gamma][delta] T lymphocytes are licensed for professional antigen presentation by interaction with opsonized target cells," The Journal of Immunology, vol.
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- Opsonized Zymosan Particle
- Opsonized Zymosan-Stimulated
- Opsonophagocytic Assay