For our study, we chose to use an athymic nude mouse
model to preclude the possibility of an immunologic rejection of the human septal cartilage.
subcutaneous xenograft tumor tissue sections (5 [micro]m) from our previous study were prepared and subjected to hematoxylin and eosin and immunofluorescence staining as described previously (Wang et al.
7] NT8e tumour cells in 100 [micro]l Hanks balanced salt solution (HBSS) were injected subcutaneously on the dorsal right flank of the nude mouse
as described earlier (8,9).
In the second part of the study, we established a nude mouse
model to study the relationship between the tumor mass and plasma EBV DNA concentration.
Animal skin, especially that of the nude mouse
, has a huge amount of percutaneous absorption.
The tumor models used were nude mouse
xenografts bearing two different human colon cancer cell lines: LS174T, containing wild type p53, and DLD-1, containing mutant p53.
1 Salmonella Typhimurium Mutants Selected to Grow Only in Tumors to Eradicate Them in Nude Mouse
Models (Robert M.