The following chemicals and reagents were used: Ampicillin (FLUKA), Isopropyl AY-D-1 thiogalactopyranoside (IPTG; SIGMA), LB broth (SIGMA), XL-1 Blue competent cells (AGILENT TECHNOLOGIES UK LTD), Agar (FLUKA), 1Kb DNA ladder (NEW ENGLAND BIOLABS), PCR nucleotide mix, Taq buffer and Taq polymerase X (PROMEGA), Primers (EUROFINS MWG OPERON), GStrap FF (GE HEALTHCARE), Phenol blue (SIGMA-ALDRICH), 10X Tank buffer (BIO-RAD), N-N-N-N- tetramethylethylenediamine (TEMED) (SIGMA), Ammonium per sulphate (APS) (SIGMA), Sugar donor UDP-Glucose (Sigma X), novobiocin
, kaempferol and quercetin (SIGMA), UDP Rhamnose (a kind gift from Prof.
epidermidis, the tests described by Bannerman (23) and MAcFaddin (24) were used: production of bound coagulase factor ("clumping") and coagulase-free production of hemolysis, pyrrolidonyl arylamidase enzyme, urease, ornithine decarboxylase, novobiocin
susceptibility and fermentation of the carbohydrates mannitol, mannose and trehalose.
Staphylococcus saprophyticus was susceptible to ampicillin, cephalexin, and trimethoprim-sulfamethoxazole and resistant to nalidixic acid and novobiocin
Escherichia coli O157 samples were enriched in tryptic soy broth (TSB), exposed to an immunomagnetic separation step and then cultured on cefixime potassium tellurite sorbitol MacConkey (CT SMAC) and Rainbow agar containing novobiocin
and tellurite (NT Rainbow) as previously described (Cooley et al.
Some observations on the incorporation of novobiocin
into Hektoen enteric agar for improved Salmonellae isolation.
For selective enrichment, Lab M's Mueller Kaufmann Tetrathionate Novobiocin
Broth (MKTTn) is formulated to inhibit the growth of Proteus species and allow Salmonellae to thrive.
For selective enrichment, proprietary Mueller Kaufmann Tetrathionate Novobiocin
Broth is formulated to inhibit the growth of Proteus species.
aureus isolates were found to be resistant to aztreonam, colistin, mecillinam, metronidazole, polymyxin B and nalidixic acid, but all were found to be sensitive to vancomycin, nitrofurantoin and novobiocin
The antibiotics used for this test were Amoxyclav, Cephalexin, Ciproflaxacin, Clindamycin, Cloxacillin, Co-Trimaxazole, Erythromycin, Tetracyclin, Ampicillin, Carbenicilllin, Cephatoxamine, Chloramphenicol, Co-Trimazine, Gentamicin, Norflaxacin, Oxacillin, Amikacin, Bacitracin, Novobiocin
, Vancomycin, Penicillin-G, Tobramycin, Cephaloridine, Kanamycin, Linomycin, Methicillin, Oleandomycin, Nalidixic acid, Sulphamethoxazole, Cefactor, Cefixime, Piperacillin and Cefoperazone.
Substrates of this pump include [beta]-lactams, [beta]-lactamase inhibitors, quinolones, macrolides, tetracyclines, chloramphenicol, novobiocin
, sulfonamides, trimethoprim and thiolactomycin (9).
6) Staphylococcus aureus infections that colonize catheters and other indwelling medical devices were treated with the antibiotic novobiocin
until drug-resistant S.
Additional biochemical tests those are useful in separating the Staphylococcus species include catalase, coagulase, growth and fermentation of mannitol salt, andresistance or susceptibility to the antibiotic novobiocin
3], 15 g de bactoagar, 5 g de KBr, 100 mg de cycloheximide, 2 mg de benomyl, 25 mg de cefalexina, 30 mg de novobiocin
, 50 mg de kasugamicina y 1 L de agua destilada), selectivos para X.
1 mL was inoculated into 10mL of RV broth (Oxoid CM 669 containing Novobiocin
35 mg/L) and incubated for 16-24h at 42[degrees]C.
mallei is usually sensitive to a variety of antibiotics including tetracyclines, ciprofloxacin, streptomycin, novobiocin
, gentamicin, imipenem, ceftrazidime, and the sulfonamides.