A study of 9 Thai patients with documented recent DENV infections found all sera were cross-reactive with ZIKV, including when tested using neutralization tests
, in the absence of current or prior ZIKV infection.
dagger]) Serum dilution-plaque reduction neutralization test
, titers of neutralizing antibodies to ZIKV, DENV-1, and DENV-2.
One limitation of this work is the virus used for neutralization tests
Moreover, researchers have been concerned that the plaque/focus reduction neutralization test
, the standard serologic assay for distinguishing different flavivirus serocomplexes, will be unable to differentiate Zika virus from DENV.
Serum samples were assessed for immunologic responses by using a virus neutralization test
(VNT) and a Luminex bead assay to the nucleocapsid protein.
for 2 of the 3 CHATV patients resulted in titers >4 times those for other studied California serogroup viruses, which met the diagnostic criterion for confirmed CHATV infection; the third CHATV patient with a lower titer likely had CHATV infection (Table 3).
The viruses were stored at -80[degrees]C until the neutralization test
Banked blood samples collected from white-tailed deer, raccoon, and (occasionally) moose (Alces alces) and coyote (Canis latrans) during 2009-2014 were analyzed by plaque-reduction neutralization test
for HRTV neutralizing antibodies by using African green monkey kidney (Vero) cell culture.
Array results were confirmed on a selection of positive and negative serum samples (n = 14 per country) in MERS-CoV neutralization tests
performed as described (2) (Table 2).
For neutralization tests
, serum were inactivated at 56[degrees]C for 30 min; duplicate 100-mL volumes of doubling dilutions were prepared in Leibowitz medium (1:10-1:640) and incubated with equal volumes of medium containing a calculated 100 50% tissue culture infectious dose virus at 37[degrees]C for 45 min, and 25 mL Vero cells (8 x [10.
We also provide evidence of WNV human infections confirmed by IgM ELISA and seroconversion by 90% plaque reduction neutralization tests
of paired serum samples obtained from persons with febrile illness and viral encephalitis in 2011.
Serum neutralization tests
were conducted as described (10) by using Vero B4 (MERS-CoV) or PT (BCoV) cells.
Serologic indication of SLEV circulation in Peru was first obtained from hemagglutination-inhibition and neutralization tests
of samples collected in 1965 from residents of eastern Peru (5).
1%) horses were positive for antibodies against flaviviruses by ELISA, and all 67 were positive for TBEV by virus-specific neutralization tests
(Table, Appendix, wwwnc.
The blot data provided evidence of an infection with DOBV; to confirm the results, we performed serotyping by using focus reduction neutralization tests