Isolation of pathogens: Surveys were conducted to collect diseased plants of Clusterbean (Cyamopsis tetragonoloba), Cowpea (Vigna unguiculata), Moth bean (Vigna aconitifolia) and Horse gram (Macrotyloma uniflorum) from Jodhpur, Jaipur and Bikaner Districts of Rajasthan during the months of August and September, 2011.
Two week old plants of arid legumes i.e., clusterbean, cowpea, moth bean and horse gram, were then inoculated with conidial suspension of specified isolate (from which it was originally isolated) at the base by disturbing the soil.
To perform analysis of molecular variance (AMOVA), the 52 isolates of root pathogens from arid legumes were divided into four populations based on the arid legume crop from which they were isolated i.e., population one (clusterbean), population two (horse gram), population three (cowpea) and population four (moth bean).
For example, population one (32 isolates of Clusterbean), population two (two isolates of horse gram), population three (eight isolates of cow pea), and population four (10 isolates of moth bean).
The data revealed that the genetic diversity of root pathogen isolates of population four (moth bean) was the richest among all the four populations.
MIXED BLACK DAL (Serves 8) | 100g black lentils, also known as whole urad dal, with skin | 100g mung beans, also known as whole mung dal, with skin | 100g chana dal, this is inner layer of black chickpeas split in half | 100g moth beans
| 2 medium onions, finely chopped | 2cm peeled root ginger, cut into slithers | 3tsp finely chopped hot green chilli | 60g unsalted butter | 13/4tsp salt | 1/2tsp turmeric | 4tsp garam masala Method The night before rinse all four types of dal and rinse them well then combine them in a heavy-based pan about 25 centimetres in diameter.
Major pulses produced include: chickpea or white gram, black gram, Moong, Masoor or Lentils, Mash, White Lobia or Cowpeas, Red Lobia or kidney beans, Urd, Arhar or pigeon peas, Matri or Lathyrus and Moth or Moth beans