We send our hepatocyte or microsome
incubation samples out to other reference laboratories for structure elucidation/identification for initial method development.
Mitochondria and microsomes
from freshly extracted mouse liver were prepared by differential centrifugation and suspended in 20 mM [K.sub.2]HP[O.sub.4] buffer containing 20% glycerol with added leupeptin, pepstatin, antipain, and PMSF as described previously [29, 30].
On stipulated day the animals were slaughtered, their livers taken out and microsomes
prepared for the estimation of activities of various membrane bound DME such as cytochrome P450 (CP), cytochrome b5 (CB), cytochrome-c-reductase (CCR), aniline hydroxylase (AH), acetanilide hydroxylase (AAH), benzphetamine demethylase (BD), aminopyrine demethylase (APD), N.N.
(22) The stoichiometry of C21-hydroxylation of 17-hydroxyprogesterone by bovine adrenocortical microsomes
was established by Cooper et al.
The partitioning of metals among the (1) nuclei and brown bodies; (2) lysosomes and mitochondria; (3) microsomes
; and (4) soluble cytosolic fraction is presented in Table 2.
Johanet et al., "Identification by proteomic tool of atypical anti-liver/kidney microsome
autoantibodies targets in de novo autoimmune hepatitis after liver transplantation," Annals of the New York Academy of Sciences, vol.
Abbreviations: WEL, wedelolactone; OH, hydroxyl group; UGT, uridine diphosphate-glucuronosyltransferase; HPLC, high-performance liquid chromatography; SPE, solid-phase extraction; NMR, nuclear magnetic resonance; UPLC, ultraperformance liquid chromatography; Q-TOF MS, quadruple time-of-flight mass spectrometry; HLM, human liver microsome
; HIM, human intestine microsome
; HKM, human kidney microsome
; UDPGA, uridine 5'-diphosphate-glucuronic acid; UV, ultraviolet; COSY, correlation spectroscopy; HSQC, heteronuclear single quantum correlation spectroscopy; HMBC, heteronuclear multibond correlation spectroscopy; ROESY, rotating frame nuclear Overhauser effect spectroscopy; IDA, information-dependent acquisition; DMSO, dimethyl sulfoxide.
Aromatization of norethindrone to ethinyl estradiol by human placental microsomes
. J Clin Endocrinol Metab.
Studies showed that the patients with AA genotype had a less fentanyl consumption to achieve pain control than those carrying GG and AG genotypes during the first 24 h postoperatively in patients undergoing lower abdominal surgery or cesarean., Moreover, in vitro study has shown reduced metabolism of fentanyl in liver microsomes
of AA genotype. Obviously, CYP3A4*1G polymorphism contributes to the variability in response to fentanyl.
Serum antinuclear antibodies, antimitochondrial antibody, and anti-liver-kidney microsomes
were negative, and he had normal ceruloplasmin levels.
We determined the glucuronidation kinetics of BPS, BPF, and BPAF experimentally in both human liver and intestinal microsomes
. We did not conduct additional experiments on BPA glucuronidation kinetics, as several well-conducted studies already exist (Coughlin et al.
He then focused on recombinant CYP preparations like bactosomes, bacterial membranes containing cytochrome P450s co-expressed with NADPH-CYP reductase, which have a higher specific activity than liver microsomes
and can be used for the production of high quantities of metabolites as drug candidates.
Based on this, two experiments were designed: (1) in vitro, the effects of genistein on rat and human liver microsomes
(RLM and HLM) and CYP2C9 recombinant enzyme were studied and (2) in vivo, the effects of genistein on pharmacokinetics of celecoxib in male SD rats were detected by ultrahigh-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method.
Stability of [[sup.18]F]FE@SUPPY was tested against mouse liver microsomes
, mouse S9 fraction, and mouse plasma (BD Sciences).