Lonza Walkersville announced its new offering of Silensomes Human Liver Microsomes
(HLM) products pooled human liver microsomes
for in vitro cytochrome P450 (CYP) phenotyping.
On stipulated day the animals were slaughtered, their livers taken out and microsomes
prepared for the estimation of activities of various membrane bound DME such as cytochrome P450 (CP), cytochrome b5 (CB), cytochrome-c-reductase (CCR), aniline hydroxylase (AH), acetanilide hydroxylase (AAH), benzphetamine demethylase (BD), aminopyrine demethylase (APD), N.
The mutagenic effects of sodium nitrite and monosodium glutamate used as food additivesdemonstrated by the Salmonella microsome
Tissue preparations and assays Preparation of liver microsomes
OBJECTIVES: The purposes of this study were to characterize the in vitro metabolism of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) by human liver microsomes
(HLM) and recombinant human CYPs, and to identify the CYP(s) that are active in the oxidative metabolism of BDE-47.
Briefly, rat liver microsomes
(Invitrogen, Carlsbad, CA) were diluted to 1 mg protein/mL in 0.
1515) note that the development of the Salmonella mammalian microsome
mutagenicity assay in the 1970s was a seminal event leading to the emergence of new approaches to toxicology based on "omics," in vitro assays, high-throughput testing, and computational methods.
The Salmonella mammalian microsome
mutagenicity assay has been central to the field of genetic toxicology since the 1970s.
As the first microsome
product line formulated to deliver different classifications of cytochrome P450 (CYP) enzyme activity--high levels for inhibitions studies (InVitroCYP H-class), moderate levels for metabolic studies (InVitroCYP M-class) and custom activity levels (InVitroCYP C-class)--Celsis InVitroCYPs now enable researchers to choose a microsome
product to optimize their specific study outcomes.
With throughputs approaching fluorescence- or radioactivity-based methods (3500 data points per eight-hour shift on a single instrument), and full compatibility with liver microsome
preparations, the RapidFire P450 inhibition assay platform enables researchers to advance lead optimization with highly accurate mass spectrometric data.
CO donor dose-dependently inactivated CYP2E1 of ethanol-incubated microsome
, which was mimicked by HO-1 substrate but abolished by CO scavenger.
Human liver cytosol and microsome
extracts were obtained from BD Biosciences (San Jose, CA).
Method for the assay of microsome
1'-hydroxy midazolam concentrations was adapted from those described by our previous report (Li et al.
Studies are most frequently performed with (cryopreserved) primary cells or tissue fractions, such as microsomes
, S9 or cytosol, derived from animal organs or human donors (Soars et al.
Lung and OM microsomes
from homozygous 2A13/[2F1-TG.