Microscopic agglutination tests
were conducted in the Laboratory for Reproductive Bacterial Diseases in the Biological Institute of Sao Paulo to detect antibodies against Leptospira spp.
Venous blood samples were tested for the presence of anti-leptospiral antibodies using microscopic agglutination test
(MAT) at the Institute of Medical Research (IMR) following standard methods .
TABLE 1: Serovar panel used in the microscopic agglutination test
. Species Serogroup Serovar L.
Improved microtechnique for the leptospiral microscopic agglutination test
The microscopic agglutination test
(MAT) is the gold standard test but requires significant expertise to maintain and interpret.
(29) Diagnosis of leptospirosis is usually retrospective because of the length of the time required for diagnosis by microscopic agglutination test
(MAT) reference methodology.
Two tests have a role in veterinary diagnosis: the microscopic agglutination test
and ELISA .
In 1988, during the peak of the monsoon season, serum and urine samples from 40 patients revealed 82.5 per cent had specific leptospiral antibodies, with titres ranging from 1:160 to 1:6400 by microscopic agglutination test
(MAT) against different serovars, and 71 per cent seropositive cases were diagnosed by MAT with clinical suspicion of leptospirosis in 1996 (4).
Assessment of the efficacy of an IgM-ELISA and microscopic agglutination test
(MAT) in the diagnosis of acute leptospirosis.
Microscopic agglutination test
(MAT) is the gold standard for diagnosis of leptospira infection in dogs (Chappel et al., 2004).