Kirshbaum, "Outline of details for microbiological assays
of antibiotics: second revision," Journal of Pharmaceutical Sciences, vol.
for vitamin [B.sub.12] with use of a colistin-sulfate-resistant organism.
Comparison of different phenotypic tests for detection of MBL producing isolates Phenotypic test Number of MBL positive isolates (n=21) Modified Hodge test on Mueller Hinton Agar 15 Modified Hodge test on MacConkey Agar 19 Combined Ceftazidime + EDTA 8 disc test Imipenem + EDTA 20 Double disc synergy test 20 EDTA-Imipenem microbiological assay
20 Extended EDTA Ceftazidime 12 disc synergy Imipenem 20 test Meropenem 18 n, number of screen positive isolates tested
for vitamin B12 performed in 96-well microtitre plates.
Comparison of three commercial kits and a microbiological assay
for the determination of vitamin B12 in serum.
(11), who pointed out the wide variability of the folate values obtained with different methods (microbiological assay
, radioassay, chemiluminescence, HPLC, and ioncapture assay), with the serum folate concentrations measured by the ACS:180 method being, on average, 61% higher than those obtained with the ion-capture assay (IMx).
The DSS eluate was considered to represent a 1:40 dilution of serum and was analyzed by microbiological assay
(2) with Lactobacillus casei subsp.
These EDTA-WB hemolysates require a further 20-fold (or more) dilution before analysis by microbiological assay
with Lactobacillus rhamnosus.
casei), used in the classical microbiological assay
of folate, would prevent the saponin molecule from reaching the outer bacterial membrane.
Serum folate was determined by a Lactobacillus casei microbiological assay
(22 ) and serum cobalamin by a L.
 Nonstandard abbreviafions: RCF, red cell folate; Hb, hemoglobin; MA, microbiological assay
; RFPB, radio-folate-binding protein; AA, ascorbic acid; PteGlu, pteroylmonoglutamic acid (folic acid); 5C[H.sub.3]-[H.sub.4]-Pte[Glu.sub.n], 5-methyltetrahydroiolic acid polyglutamates; 5C[H.sub.3]-[H.sub.4]-PteGlu, 5-methyltetrahydroiolic acid monoglutamate; DPG, 2,3-diphosphoglycerate; T-, tense; and R-, relaxed.
Traditionally, serum and erythrocyte folate were analyzed by microbiological assay