DNA from environmental and clinical isolates
was subjected to PCR using primers specific for virulence genes.
tested were cultured on blood agar overnight.
Analysis for rpoT genotyping of 64 samples collected in the west and southwestern areas revealed that 46 isolates
out of 64 isolates
were 4 copy type and 18 isolates
were 3 copy type (Table 1).
Previous studies have found that PFGE is a good measure of genotypic relatedness between Enterobacter sakazakii isolates
Phylogenetic evidence of widespread distribution of genotype 3 JC virus in Africa and identification of a type 7 isolate
in an AIDS patient.
collected annually ranged from 3,128 to 3,961 (Table).
From the artificially inoculated and Alternaria blight diseases safflower leaves the test isolates
were reisolated separately on PDA medium and incubated at 28 [+ or -] 2 0C.
Since oxacillin resistance has become a major problem in hospitals which leaves few therapeutic options and since the presence of pandemic clones indicates clonal dissemination of MRSA isolates
, the aim of the present study was to perform molecular characterization of MRSA strains isolated from the University Hospital of the Botucatu School of Medicine (HCFMB), UNESP.
We detected isolates
of genotypes A, B, C, and a new genotype similar to the C.
Overall, most of the bacteria that were found in the isolates
were Escherichia coli (54.4%), followed by Klebsiella pneumoniae (11.8%), Enterococcus species (7%), Proteus mirabilis (5.1%), Pseudomonas aeruginosa (2.9%), Staphylococcus aureus (2.3%), and Enterobacter cloacae (2.2%), he commented.
During March through May 1989, coxsackievirus B5 was the most commonly reported NPEV (16 [52%] of 31 isolates
Researchers at the University of California at San Francisco said last week that the later isolates
killed more white cells and multiplied faster than those taken from patients early in infection.
Additional characterization of the isolates
from this study was not performed; however, USA300 has previously been reported to carry staphylococcal cassette chromosome mec (SCCmec) type IVa and classified as sequence type 8 (ST8) by multilocus sequence typing (4,5).
Nonpolio enterovirus (NPEV) surveillance data show that isolates
from March through May predict the types likely to be isolated in July through December, which includes the peak enterovirus season (1).
In the second study, it apparently was a problem of too many virus isolates
rather than too few.