positive for sdrC, sdrD, and sdrE determined by PCR and DNA sequencing were used as control strain for every PCR assay for the detection of sdrC, sdrD, and sdrE.
Genotypic characterization of Enterobacter sakazakii isolates
by PFGE, BOX-PCR, and sequencing of the fliC gene.
collected annually ranged from 3,128 to 3,961 (Table).
Researchers could also use the viral isolate
to study the disease in animal models.
carthami test isolates
was prepared separately from 10 days pure culture in plates, by flooding with 5-10 ml sterile distilled water.
Since oxacillin resistance has become a major problem in hospitals which leaves few therapeutic options and since the presence of pandemic clones indicates clonal dissemination of MRSA isolates
, the aim of the present study was to perform molecular characterization of MRSA strains isolated from the University Hospital of the Botucatu School of Medicine (HCFMB), UNESP.
We detected isolates
of genotypes A, B, C, and a new genotype similar to the C.
During March through May 1989, coxsackievirus B5 was the most commonly reported NPEV (16 [52%] of 31 isolates
Additional characterization of the isolates
from this study was not performed; however, USA300 has previously been reported to carry staphylococcal cassette chromosome mec (SCCmec) type IVa and classified as sequence type 8 (ST8) by multilocus sequence typing (4,5).
By studying a virus responsible for feline leukemia, researchers at the Harvard School of Public Health in Boston and at Colorado State University in Fort Collins found that procedures used to separate viruses may fail to isolate
"acutely pathogenic" (disease-producing) virus strains while selecting for less virulent viruses.
The Photorhabdus isolates
recovered from nematodes and from the patient's hand were compared on the basis of nucleotide sequences of 2 housekeeping genes, glnA and gyrB.
Sequence analysis indicated that the new H5N1 isolate
(A/duck/Laos/3295/2006) (GenBank accession no.
their immunosuppressive candidate, Andrew V.
Given that the European EV75 isolate
grows easily in a variety of cell lines, is detected by common EV genus-specific antibodies, and that EV surveillance and typing were performed in Spain since 1988 (2), EV75 might have begun to circulate in Spain recently.
A resistant isolate
that was untypable by conventional serotyping (rough) but that had a pulsed-field gel electrophoresis (PFGE) profile associated with serotype Kentucky, was included in this study.