Hypersegmented Neutrophil in Cases and Control Population HSN Cases Control Total Present 67 11 78 Absent 33 89 122 Total 100 100 200
Deficiency of the maturation factors also affects the WBCs which can be detected by the presence of hypersegmented neutrophils in the peripheral smear.
Present study intends to compare the Mean Corpuscular Volume and Hypersegmented neutrophils in 100 alcoholics and 100 non-alcoholics.
However, the possibility of vitamin B12 deficiency was also entertained in view of relatively mild thrombocytopenia, extremely elevated LDH level, and presence of hypersegmented neutrophils on blood smear.
Vitamin B12 deficiency causes dyssynchrony between nuclear and cytoplasmic maturation, resulting in ineffective erythropoiesis, intramedullary hemolysis, and characteristic features of megaloblastic anemia on blood smear: macrocytosis and hypersegmented neutrophils .
The hypersegmented neutrophils
indicated that the neutrophils on the peripheral blood film showed six to eight segments per neutrophil rather than the typical 3-5 as most mature neutrophils would have.
Six (20%) out of 30 were having thrombocytopenia and 5 (16.66%) patients were found to have hypersegmented neutrophils
 Hypersegmented neutrophils
were seen in 40% cases and peripheral blood smear showed dimorphic anaemia in 20% cases.
Once the patients were selected, various hematological parameters including peripheral smear analysis for megaloblasts, macrocytes, hypersegmented neutrophils and RBC indices were carried out.
There were nine patients in our series that had Vit [B.sub.12] above 200pg /ml--six were between 200 and 300 pg/ml and nine, macrocytosis with hypersegmented neutrophils were seen in six patients and raised LDH (sign.
Megaloblastosis is characterized by many large immature and dysfunctional red blood cells (megaloblasts) in the bone marrow [Figure 4] and also by hypersegmented neutrophils
having five or more nuclear lobes in more than 5% of cells in the peripheral smears