hybridoma


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  • noun

Words related to hybridoma

a hybrid cell resulting from the fusion of a lymphocyte and a tumor cell

References in periodicals archive ?
Department of Hybridoma, Monoclonal Antibody Research Center,
After fusion of splenocytes with the hybridoma cells, we obtained eight positive clones.
We found that the staining of the NE as well as the cytoplasmic staining was completely abrogated after depletion of mAb K81-116-6 from the hybridoma supernatant by incubating the supernatant with nitrocellulose membrane strips carrying GST-Nesprin2-SMC or with Glutathione-Sepharose 4B beads carrying GST-Nesprin-2-SMC.
After single cell subcloning of the hybridoma cells, specific clones were expanded in culture and hybridomas were stored in liquid nitrogen.
In addition, all hybridoma supernatants were tested against HEK cell antigens (negative control) at a concentration of 0.0025 ng/well.
Galactose, fructose and maltose significantly increased protein production by the factor of 1.7, 2.0 and 2.2, respectively.The study extends understanding of augmented use of superior carbohydrate in cell culture media to improve growth of hybridoma cells and subsequently higher yields of antibody.
For immunocytochemistry, the following antisera were used: rat monoclonals against VE-cadherin (11D4.1, BD Biosciences), PECAM-1 (MEC 13.3, Santa Cruz), and E-Cadherin (DECMA-1, Santa Cruz), mouse monoclonals against cardiac Troponin T (CT3, Iowa Hybridoma Bank), Isl1 (39.4D5, Iowa Hybridoma Bank), Oct3/4 (C-10, Santa Cruz), SMA (Neomarkers), N-cadherin (clone 3B9, Invitrogen), MyHC (MF20, Iowa Hybridoma Bank), and aactinin (Clone BM-75.2, Sigma), goat polyclonals against GATA4 (C-20, Santa Cruz) and Isl1 (GT15051-100, Acris Antibodies), rabbit monoclonals against MEF2c (D80C1) and VEGF receptor 2 (Flk1) (55B11) from Cell Signaling, and rabbit polyclonals against EGFP (kindly provided from Dr.
The cells were blocked and incubated overnight with the murine monoclonal antibody MHC obtained from hybridoma (MF20, 1: 5 v/v; Developmental Studies Hybridoma Bank, University of Iowa), which is able to recognize the sarcomeric myosin expressed by differentiated cardiomyocytes.
Anti-pGHR monoclonal antibodies were prepared using the hybridoma technique as previously described (Lan et al., 2014).
(2008) Preparation of antibody against horseradish peroxidase using hybridoma technology.
Prior to this, she was technical sales and marketing manager for Harvard Bioscience, where she doubled sales over a three-year period and improved market share by developing an effective technical marketing strategy for platform hybridoma fusion systems and cell transfection equipment.
(ii) Plant or animal cell cultures (including those resulting from recombinant DNA or hybridoma techniques),
destructans-positive hybridoma supernatant serving as a positive control or 50 [micro]l of mouse serum serving as a negative control, or 25 [micro]l of the unknown bat sera diluted 1:2 in blotto was added to each well and allowed to incubate at 37[degrees]C for 1 hr.
Its service portfolio includes mouse and rat monoclonal antibody production using hybridoma technology, human, monkey, rabbit, chicken, dog, llama and camel monoclonal antibody production using various antibody library technologies.