Isolation of pathogens: Surveys were conducted to collect diseased plants of Clusterbean (Cyamopsis tetragonoloba), Cowpea (Vigna unguiculata), Moth bean (Vigna aconitifolia) and Horse gram (Macrotyloma uniflorum) from Jodhpur, Jaipur and Bikaner Districts of Rajasthan during the months of August and September, 2011.
Two week old plants of arid legumes i.e., clusterbean, cowpea, moth bean and horse gram, were then inoculated with conidial suspension of specified isolate (from which it was originally isolated) at the base by disturbing the soil.
To perform analysis of molecular variance (AMOVA), the 52 isolates of root pathogens from arid legumes were divided into four populations based on the arid legume crop from which they were isolated i.e., population one (clusterbean), population two (horse gram), population three (cowpea) and population four (moth bean).
For example, population one (32 isolates of Clusterbean), population two (two isolates of horse gram), population three (eight isolates of cow pea), and population four (10 isolates of moth bean).
The largest distance was recorded between population two (horse gram) and three (cowpea) and the least between the population one (Clusterbean) and population four (moth bean).
According to Ayurveda, "It results in serious occlusion of body channels." Radish, garlic, moringa, basil, horse gram
, black gram, mango, pomegranate and all sour liquids are incompatible with milk.