Each plasma sample (100 [micro]L) was added with two internal standard solutions (10 [micro]L of 4-chlorophenyl-alanine in water, 0.3 mg/mL; 10 [micro]L of heptadecanoic acid
in methanol, 1 mg/mL) and with 300 [micro]L methanol/chloro-form (3:1) to extract the metabolites.
GC-MS analysis of Fatty acid methyl esters in cell wall of isolate SKCMM1 Retention Compound name time 19.01 12-Methyltetradecanoic acid 19.62 Pentadecanoic acid, methyl ester 21.64 Hexadecanoic acid, methyl ester 23.03 Heptadecanoic acid
, methyl ester Retention Mol.
Additionally, an internal standard (100 [micro]l) containing heptadecanoic acid
(C17:0 FFA), 1,2-diheptadecanoin (C17:0 DAG), and triheptadecanoin (C17:0 TAG) was added.
, N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), and methoxyamine hydrochloride were obtained from Sigma-Aldrich (St.
This saturated fat is called heptadecanoic acid
Fatty acids Percentage Myristic acid 0.2090 Palmitic acid 12.84 Palmitoleic acid 0.2812 Heptadecanoic acid
0.1047 Stearic acid 3.3884 Oleic acid 22.5836 Linoleic acid 58.2370 Arachidic acid 0.2250 Eicosenoic acid 0.3839 Behenic acid 0.0356 Lignoceric acid 0.0414 Table 2 Chemical compositions of volatile oil component of N.
There were nine (38.30%) moderately active antimicrobial compounds: 9.92% of phytol, 9.75% of 10-octadecenoic acid methyl ester, 5.46% of n-hexadecenoic, 5.27% of 9-hexadecenoic acid, 3.48% of octadecanoic acid methyl ester, 1.22% of heptadecanoic acid
methyl ester, 1.14% of eicosanoic acid methyl ester, 1.04% of 9-hexadecenoic acid methyl ester (Z)-, and 1.02% of squalene.
The most dominant SAFAs were palmitic acid, pentadecanoic acid, stearic (octadecanoic) acid, tetracosanoic acid, and heptadecanoic acid
. Twenty (20) of the 27 unsaturated FAs were PUFAs and 7 were MUFAs.
(C17:0)###0.010+-0.040 b###0.018+-0.040 b###0.214+-0.040 a###0.026+-0.040 b###0.026+-0.040 b
Among minor components occuping less than 2% of fatty acids, C15:0 (pentadecanoic acid) and C17:0 (heptadecanoic acid
) existed both in PL and TG fractions, but C20:4n-6 and C20:5n-3 were hardly detected in TG.
Thereafter, the solvent was evaporated and 1.0 mg of heptadecanoic acid
in hexane was added as an internal standard.
Hens fed 1.6% CLA had significantly higher lauric acid, myristic acid, palmitic acid, heptadecanoic acid
, stearic acid, and arachidic acid in egg yolk compared with the controls, while the content of behenic acid content was significantly less.
Identification of the peaks has been achieved by comparing the time length of their retention with those of the pure standard compounds (Sigma) and the quantification of acids has been carried out in relation to heptadecanoic acid
methylic ether as internal standard.
(E17:0) ethyl ester in hexane (10 mg/L) was used as the internal standard.
Ethylation for fatty acid analysis: A total of 1 mL of medium to which 1 mg of heptadecanoic acid
([C.sub.17:0]) (H3500, Sigma, St.