Germinally transgenic tadpoles were produced by restriction enzyme-mediated integration nuclear transplantation according to Kroll and Amaya (1996), with the following modifications: sperm was purified by centrifugation on a two-layer discontinuous Percoll (Sigma) gradient before the permeabilization step, which was performed with digitonin (Sigma) instead of lysolecithin.
Before photographing, germinally transgenic tadpoles NF stage 52 were anaesthetized in 0.
0] germinally transgenic tadpoles were selected and bred to obtain F1 tadpoles.
The tensions among the distinct relations of rule manifested in the proto-society of the household, while prior in generation and time to the polis and not formally or constitutionally political, germinally or potentially evidence the fundamental ontological relations which in one manner or another may come to be predominant and reveal the constitution of a given polis.
If all constitutions are virtually or germinally within the relations of rule held in tension within the familial household, and if all just relations within that complex unity originate in generation and time through the royal and political rule between husband and wife, though in reference to offspring it is intended by nature to constitute an order of arete or aristocratic measure, arguably it is therein that one may discern the intimation of politeia as essentially constituted and achieved within a polis.