The feeder layer
of human embryonic fibroblasts supports the growth of human spermatogonial stem cells].
Mesenchymal stem cells as an appropriate feeder layer
for prolonged in vitro culture of human induced pluripotent stem cells.
In the present study we have described two methods to isolate hair follicle cells and then grow them for specific period of time in serum based medium without feeder layer
A recent study reported that type of stromal feeder layer
used in LTC-IC LDA affects the determination of LTC-IC frequencies in uncultured cells and also has a significant effect on cultures.
Previous studies have shown that human limbal epithelial progenitor cells can be expanded and maintained on intact, but not denuded HAM or plastic in the absence of 3T3 murine fibroblast feeder layers
After electroporation, mESCs were allowed to spread on pre-coated gelatin-plastic flasks containing mitomycin C-inactivated feeder layer
of primary cultures of mouse embryonic fibroblasts (MEF) in ESC medium containing G418 (Sigma, USA).
Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers
Interpretation & conclusions: The limbal epithelial cells cultured in the presence of mitomycin C treated 3T3 feeder layer
were able to maintain the expression of putative stem cell markers.
To research this, the scientists generated female iPS cells on feeder layers
without LIF and found that one of the X-chromosomes in each iPS cell remained silent.
1% Gelatin coated 4-well multidish (Falcon BD) with or without mitomycin C-treated STO cell feeder layer
and cultured at 37[degrees]C in a humidified atmosphere of 5% C[O.
1 mM [beta]-mercaptoethanol on a mytomycin-C (Roche, Mannheim, Germany) inactivated murine embryonic fibroblast (MEF) feeder layer
Influence of feeder layer
type on the efficiency of isolation of porcine embryo-derived cell lines.
Kidson discusses, namely where feeder layers
are used to plate the inner cells of a blastocyst, is very risky in any human stem cell work.
Within 4-6 days, embryos attached to the feeder layers
and formed ICM outgrowths with prominent nucleoli and dense morphology.
Other than increasing the potential to guide stem cells to create desired materials for research and clinical applications, using nanoscale topographies could eliminate (or alternatively enhance) steps including those involving feeder layers
and synthetic induction supplements currently used in stem cell culture.