Fertilization of C57BL/6 mouse sperm collected from cauda epididymides
after preservation or transportation at 4[degrees]C using laser-microdissected oocytes.
The changes in several absolute organ weights in male groups (heart, brain, kidneys, spleen, testes, and epididymides
) showed dose-unrelated, statistically significant increases with no associated changes in gross pathology or histopathology; thus, they were considered incidental findings.
Spermatozoa collected from cauda epididymides
were resuspended in prewarmed (37[degrees]C) phosphate-buffered saline (PBS) containing 200 nM MT-DR FM and incubated at 37[degrees]C for 45min.
Computer-assisted sperm analysis (CASA) was performed on an IVOS instrument (Hamilton Thorne, Inc.) using sperm from one of the cauda epididymides
taken from mice at the time of necropsy.
Immediately after bucks were slaughtered, the testes with epididymides
of each animal were collected and put into plastic bags labeled with animals particularities and transported in an ice pack to the laboratory where the epididymides
were separated from testes and then testicular and epididymal parameters including testicular weights (TW, g), testicular diameter (TD, cm), testicular length (TL, cm), testicular volume (TV), and epididymal weights (EW, g) were measured and recorded for each animal according to methods and procedures adopted from Oyeyemi et al.
To evaluate the effect of mitochondrial uncoupling by FCCP, spermatozoa from 3 bovine epididymides
(n = 3) were collected and diluted to a concentration of 100 million spermatozoa per mL in modified TALP.
were rinsed in ice-cold 0.01 M phosphate buffer (pH 7.4) and kept at -80[degrees]C until used.
Three months later, the animals were sacrificed, and the testicles and epididymides
adhering to the testicles were removed for histological examination.
Sperm density of the testis and cauda epididymides
was decreased significantly (P [less than or equal to] 0.001) in rats treated with the complexes at all the dose levels (Table 3).
Both testes and their epididymides
did not show abnormal findings.
The excised epididymides
were cut into small pieces and placed in F12 media (1 ml) containing 0.1% bovine serum albumin prewarmed to 34[degrees]C and incubated for 15 min to facilitate sperm transmigration from the epididymis.
Canine spermatozoa were collected from caudal epididymides
. Sperm motility, morphology, plasma membrane integrity, and acrosome integrity were evaluated immediately after sperm recovery.
, and seminal vesicles were dissected and weighed (1 mg) (Hijazi et al., 2012).
Histopathological Evaluation of Soft and Hard Tissues: The hard tissues (right femur) and soft tissues, vital organs (kidneys, liver, heart, spleen, adrenals, and lungs), reproductive organs (testes, prostate, epididymides
, and seminal vesicle) and samples of skeletal muscles were collected, fixed with 10% formalin and later processed according to standard laboratory protocols .