Caption: Figure 4: Flowchart of cell binarisation: (a) grey histogram of I-channel in cell image, (b) edge gradient
image of cells, (c) image of cell contour through a gradient threshold, (d) I-channel cell image modified by cell contour, and (e) binary image of cell.
Therefore, we propose the MOORGB which uses multiobjective optimization method (maximizing the difference between target and background, improving the uniformity within the target region, considering the edge gradient
) to improve the segmentation performance.
For each pixel the local edge gradient
magnitude is estimated with the maximum response of all 8 kernels at this pixel location.
The edge gradient
features are commonly extracted by a series of differential operators based on derivative information.
The method representing the shape via edge gradient
direction is more operable and feasible, as it requires less prior-period image processing works.