Postmortem research by Ashley Bush, Ph.D., a senior scientist at Prana and coauthor of the pilot study, involved the use of strong chelators such as edetic acid
to break up the plaques and access the amyloid beta proteins.
The culprit is an antibody that clumps platelets in the presence of the edetic acid
contained within purple-topped collection tubes.
Estimation of glomerular rate from plasma clearance of 51-chromium edetic acid
. Arch Dis Child 1972;47:613-7.
This activity was lost when the enzyme extract was preincubated with 10 mM of edetic acid
and was partially restored by addition of 1 mM Zn[Cl.sub.2], indicating the presence of a metallo-carbapenem hydrolyzing [Beta]-lactamase.
Agents found to be ineffective in randomized clinical trials include nifedipine, fish oil supplements, edetic acid
chelation therapy, ketanserin, suloctidil, and naftidrofuryl.