LpxK is the kinase that phosphorylates the 4'-hydroxyl of the distal glucosamine of lipid A disaccharide
to form lipid [IV.sub.A] [67, 68].
Following concentration and dialysis, UTI fraction was diluted with 0.5 M ammonium acetate 5X buffer, pH8.0, and incubated overnight at 37[degrees]C with 0.025U of Chase-ABC (Sigma-Aldrich, MO, USA), allowing for chondroitin sulphate complete depolymerisation into constituent disaccharide
units with an unsaturation between C4 and C5 of hexuronic acid ([DELTA]-disaccharides).
Sucrose, lactose, and maltose are common disaccharides
in the market.
Chondroitin sulfate (CS) is a glycosaminoglycan (GAG) composed of repeating disaccharides
of glucuronic acid and N-acetyl-galactosamine, which are variously substituted by sulfate groups.
By considering all stereochemical alternatives, eleven isomers can be produced when a disaccharide
is formed, 120 for a trisaccharide, and 1424 for a tetrasaccharide (Fig.
It is important to note that the flavored spicy rum, Scotch liqueur and the sparkling wine samples contain sucrose, which is the disaccharide
of glucose and fructose.
Very recently, we reported the synthesis and comparative antimicrobial studies of a number of furanose, pyranose and disaccharide
acetates , and acylates .
Tomatsu and coworkers studied the enzymatic degradation of glycosaminoglycans in DBS and measured a disaccharide
derived from chondroitin sulfate, and 2 disaccharides
derived from heparan sulfate (65).
The presence of mono- (fructose and glucose) and disaccharide
(sucrose) in all the plants under study was proved through the TLC analysis.
Acarbose inhibits both alpha amylase and the other alpha-glucosidases, thus preventing absorption of starch and other carbohydrates from the brush border of the intestine.2 Voglibose3 and miglitol4 inhibit the disaccharide
-digesting enzymes well, but have no effect on the starch digesting enzyme alpha amylase.
Trehalose is a nonreducing disaccharide
of glucose, naturally produced, and accumulated in many living organisms, but not in mammals .
area of the chromatograms is always well resolved from the areas containing longer chain GOS, thus enabling the separation of GOS matching one of the CODEX fibre definitions from the GOS that does not.
* The synthetic disaccharide
lactulose, consisting of fructose and galactose.
The TFD (Thomsen-Friedenreich disaccharide
) antigen in the fish protein is hidden in normal human cells but is exposed on the surface of cancer cells and is believed to play a key role in how cancer spreads.