cloning

(redirected from directional cloning)
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  • noun

Words related to cloning

a general term for the research activity that creates a copy of some biological entity (a gene or organism or cell)

References in periodicals archive ?
A quikchange-like method to realize efficient blunt-ended DNA directional cloning and site-directed mutagenesis simultaneously.
AE016958 (tag MAP 0862 and MAP 1087 coding for hypothetical proteins) and also the information about multiple cloning sites of the expression vector pQE-30, restriction sites BamHI and PstI were incorporated into the oligonucleotide primers to facilitate directional cloning. The resulting plasmid pQE501 containing an open reading frame encoding successively 6X histidine polypeptide, 18.5kDa.
It enables directional cloning of double-strand DNA using TOPO-charged oligonucleotides by adding 3` overhang to the incoming DNA (Cheng and Shuman, 2000).
Lv, and W.Wu (2010).A QuikChange-like method to realize efficient blunt-ended DNA directional cloning and site-directed mutagenesis simultaneously.
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