, acetone, methanol, and water were used to extract secondary metabolites from the whole plant [14,15].
(1100 ul) was added, further vortexed for 5 min, centrifuged for 15 min at 9000 rpm, organic layer collected and was evaporated under the gentle stream of nitrogen.
The team examined the sensitivity of future stratospheric chlorine and ozone levels to sustained dichloromethane
The resulting crude ethanol extract was then subjected to sequential solvent partitioning to give hexane, ethyl acetate, dichloromethane
, and aqueous extracts.
Curcumin was isolated using dichloromethane
according to the procedure of Anderson et al.
was added to the reaction mixture, filtered the catalyst, and washed with dichloromethane
and used for the next reaction.
The hexane and ethyl acetate fractions from the partitioning of the leaf ethanolic extract and the dichloromethane
and hexane fractions from the partitioning of the branch ethanolic extract of A.
Dr Stephen Montzka from the NOAA added: "The increases observed for dichloromethane
are striking and unexpected; concentrations had been decreasing slowly in the late 1990s, but since then have increased by about a factor of two at sites throughout the globe.
[CASRN (Chemical Abstract Services Registry Number) 75-09-2] (or methylene chloride) is an organic solvent that has been used extensively in industrial settings (e.
and ethyl acetate fractions of three plants were redissolved in acetone and water (1:1) and hydroalcoholic fractions were redissolved in ethanol and water (1:1) at 1% (10,000 ppm) (weight by volume).
Chloroform and dichloromethane
were the organic solvents.
Reactivity results show that 80% of the degradation of CT resulted in the formation of dichloromethane
and chloroform; compared to 20% of reactivity for commercial ZVI.
More commonly known as dichloromethane
, exposure to it in high enough concentrations can cause lightheadedness, nausea and vomiting.
(DCM), hexane, methanol (MeOH), 2, 2--diphenyl--1--picril--hydrazyl (DPPH), ascorbic acid, dimethyl sulfoxide (DMSO) and ethanol were purchased from Sigma-Aldrich, USA.
africanum; namely, dichloromethane
, methanol and methanol-water.