A virtual pyrogram for hypothetical DNA sequence ACGT, where the polymerase chain reaction product bottom-strand with annealed top-strand sequencing primer is shown on the left, shaded box, and the extended nascent strand after addition of deoxyadenosine
triphosphate (dATP) and deoxycytidine triphosphate are shown in the right, shaded box.
This enzyme normally converts a molecule called deoxyadenosine
into another molecule called inosine.
The adduct of GSCH2OAc and deoxyadenosine
was identified as S-[l-(N7-deoxyadenosinyl)methyl]GSH.
This defect causes toxic levels of adenosine and deoxyadenosine
to accumulate in the blood, killing the germ-fighting B and T cells (lymphocytes).
32] P radioisotope of deoxyadenosine
5'-triphosphate and a commercial kit (Gibco/BRL, Grand Island, NY).
deoxynucleotide triphosphates (dNTPs), which are the nucleotides deoxyadenosine
triphosphate (dATP), deoxyguanosine triphosphate (dGTP), deoxycytidine triphosphate (dCTP), and deoxythymidine triphosphate (dTTP) that compose DNA.
In the disease, the lack of ADA enzyme allows the buildup of 2' deoxyadenosine
triphosphate, a chemical that is particularly detrimental to immune system cells, especially T cells.
2]-deoxyguanosine adduct when mismatched with deoxyadenosine
in DNA," Chemical Research in Toxicology, vol.
5 mM magnesium chloride, 200 [micro] Meachof deoxyadenosine
triphosphate, deoxycytidine triphosphate, deoxyguanosine triphosphate, and thymidine monophosphate.
We also investigated an alternative derivatization approach generating the pentafluoro triacetate (PFTA) derivative of adenosine, modifying the protocol previously described for deoxyadenosine
This process undergoes deoxyadenosine
methylase (Dam) dependent methylation of GATC sequence in E.
The study authors concluded that "clofarabine is the first deoxyadenosine
analog with promising activity in pediatric ALL, pediatric AML and adult acute leukaemia at doses that are well tolerated.
Because of limitations of diagnostic modalities in Pakistan, blood specimen of patient was sent to Duke University Medical Center, North Carolina, where ADA (adenosine deaminase), PNP (purine nucleoside phosphorylase), dAXP (total deoxyadenosine
nucleotide) and %dAXP (dAXP/AXP+dAXP) levels were tested.
Malondialdehyde, one of the most abundant carbonyl products, can react with DNA to form adducts with deoxyguanosine, deoxyadenosine
, and deoxycytidine that are mutagenic in bacterial and mammalian cells.
5 mmol/L magnesium chloride (Perkin-Elmer); 200 pmol/L each of deoxyadenosine
triphosphate, deoxycytidine triphosphate, deoxyguanosine triphosphate, and deoxythymidine triphosphate (Sigma); 200 pmol of each primer; and 10 [micro]L of the extracted DNA sample.