Development of bovine oocytes matured, fertilized and cultured in serum-free chemically defined medium
Nutritional studies require the use of a defined medium
(MSM or sMSM), whereas studies on physical conditions or toxins can use defined media or the undefined pea medium.
Effect of fetal bovine serum glycoproteins on the in vitro proliferation of the oyster parasite Perkinsus marinus: development of a fully defined medium
Furthermore, acidic pH promoted growth and development of early embryos cultured ex ovo in Helisoma defined medium
With this study, we aimed to develop a serum-free adipocyte/EC co-culture system based on a completely defined medium
faecium Y31 and production of Enterocin Y31 in a defined medium
in a fermenter were compared with those in MRS (reference medium) used for the optimisation of the growth of E.
Birth of piglets derived from porcine zygotes cultured in a chemically defined medium
. Biology of Reprod.
The amino acid utilization by phase I Bordetella pertussis in a chemically defined medium
. Journal of general Microbiology.
Bovine blastocysts development after in vitro maturation in a defined medium
with epidermal growth factor and low concentrations of gonadotropins.
The resulting pellet was diluted in 5 mL of modified defined medium
(mDM) [12,18,19] and then centrifuged at 1400 rpm for 5 min.
All subsequent experiments, therefore, used a final oyster homogenate concentration of 0.25 mg [mL.sup.-1] in defined medium
Te chondrogenic medium was a defined medium
consisting of DMEM-HG supplemented with 1% [ITS.sup.+] Premix, 37.5 [micro]/mL ascorbate-2-phosphate, [10.sup-7] M dexamethasone, and 10ng/mL TGF[beta]-1.
"It is also the first defined medium
to allow for long term single-cell passage," said the paper's senior author, Hong Wu, a professor of molecular and medical pharmacology at the David Geffen School of Medicine at UCLA.
Influence of epidermal growth factor and transforming growth factor-a on in vitro maturation of cumulus enclosed bovine oocytes in defined medium
. J Reprod Fertil 100: 439-446.
Blastocysts were obtained when oocytes penetrated in vitro were cultured in a chemically defined medium
. The proportions (83-84%) of oocytes that reached the 2-cell stage were not different in the presence or absence of glucose in the fertilization medium.