decalcify

Apart from having a decalcifying effect, alkali citrates also have some other beneficial properties like prevention of the formation of calcium crystals (8-12), regulation of calcium metabolism and some anti-inflammatory properties (13-15) that leads us to consider alkali citrates as possible front line fighters in the fight of the removal and/or prevention of calcification on the native human heart valves.

Paraffin-embedded constructs were decalcified (decalcifying solution, Thermo Scientific[TM], Richard-Allan Scientific, Waltham, MA, USA) for 24 h at room temperature, after which the paraffin was melted again to allow better impregnation of the decalcified constructs.

Reference laboratories have no control over the preanalytic processing of specimens, such as the use of decalcifying solutions that degrade nucleic acids or the amount of time in formalin fixative, which cross-links, modifies, and breaks nucleic acid strands, often adversely affecting DNA amplification.

After fixing samples were placed into decalcifying solution that is 5% Nitric acid for 18 -24 hrs4.

The decalcifying efficacy of these acids and chelating agents depends on the root length, duration of application time, diffusion in the dentin and the pH of the solution.

This might be due to the interaction of TPEN with other metals like [Zn.sup.2+], [Fe.sup.2+], [Mn.sup.2+] as the author described and that is why we concluded that TPEN on its own was inadequate in preventing calcification on pericardial bioprosthetic materials but could be used as an adjuvant with other decalcifying agents.

Follow these basic maintenance tips: Remove the grind immediately and clean coffee stains from the heating plate and plastic parts, clean permanent filters thoroughly after each use, decalcify every four to six weeks, and remove any permanent filters and filtration cartridges from the machine before decalcifying it.

A comparison of these urea extracts (Andersen, 1991, 1999; Kragh et al., 1997; Nousiainen et al., 1998) with the extracts obtained with decalcifying solvents alone (Shafer et al., 1994, 1995; present results) indicates that proteins preferentially extracted in neutral or acid solvents without denaturants tend to be of high MW and glycosylated, whereas proteins extracted in strongly denaturing conditions tend to be of low MW and non-glycosylated.

Decalcifying solutions vary in their effects on retention and integrity of nucleic acids and proteins.

The tissue was then decalcified in a solution of 0.7 g/1 ethylenediaminetetraacetic acid, tetrasodium; 8 mg/l sodium potassium tartrate; 99.2 ml/1 hydrochloric acid: 0.14 g/l sodium tartrate (S/P Decalcifying Solution, Baxter Scientific Products, McGaw Park, IL) and then washed in water for 2 h.

Follow these basic maintenance tips: Always remove the grind immediately after brewing, remove coffee stains from the heating plate and plastic parts, clean permanent filters thoroughly after each use, decalcify every four to six weeks, and remember to remove any permanent filters and filtration cartridges from the machine before decalcifying it.

Follow these basic maintenance tips: Once the coffee is brewed, remove the used grinds immediately; remove coffee stains from the heating plate and plastic parts; clean permanent filters thoroughly after each use; decalcify every four to six weeks; and remove any permanent filters and filtration cartridges from the machine before decalcifying it.

This unique heating system eliminates any contact with aluminum, dramatically reduces decalcifying, and brews up to 50% more quietly than most other coffee makers.