decalcify

Knees were fixed with 4% formaldehyde, immersed in OSTEOSOFT decalcifier solution for 5 wk at room temperature, and embedded in paraffin.

Sectioning supplies Wax cups marked in 2 oz Increments Tongue depressors Strips of paper - 1 cm wide (to label blocks) Dried, cleaned vertebrae Silicone molds Polyester resin Forceps Probe Paper towels Scalpel Scissors Precision saw with diamond blade Dissecting microscope Ruler Calipers Staining supplies Histology tissue cassettes Large beakers Graduated cylinders Containers to store reagents Funnel Glass slides Cover slips Slide boxes Hot plate Orbital shaker or stir plate with bars Stopwatch Small and large forceps Scalpels Flammable chemical disposal contamer Olssectlng microscope PlastiC tub Trays Paper towels Distilled water Modified Hams hematoxylin Gelatin Glycerol Listerine Hydrochloric acid (12 M) Ethanol (100%) RDO Rapid Decalcifier

Despite the fact that we have a decalcifier and an osmosis system, there was no way I could persuade the Spanish to touch tap water.

Each humerus was bisected, immersed in Decalcifier II[R] (Surgipath Medical Industries, Inc., Richmond, IL) for 25 minutes (Castanet et al., 1996), mounted, dehydrated, embedded in paraffin and sectioned through the diaphysis.

Beauty therapists assured me that having my legs rubbed with a hard-bristled hair brush and massaged with a "decalcifier" - a little vibrating vacuum cleaner-like gadget - could dispel cellulite.

Tissue samples were fixed in 10% buffered formalin, followed by decalcification with Cal-Ex Decalcifier solution (Fisher Scientific, Waltham, MA, USA) for 3 days.

Mouse joint tissue was fixed in 4% paraformaldehyde, decalcified in EDTA bone decalcifier, and embedded in paraffin.

Processing was accomplished by fixing the whole specimen in 4% formaldehyde for 72 hours and then placing it in a slow-acting decalcifier (EDTA) until optimal demineralization was obtained.