bone

Also, in using untainted, not decalcified bone we were able to rebuild the network of unshrunkblood vessel inside the auditory ossicles and could distinguish cartilage areas and blood vessels.

Status of osteoblast apoptosis in decalcified bone sections of various treatment groups was investigated by immunostaining with Runx-2 (osteoblast marker) followed by TUNEL staining (indicative of DNA fragmentation).

Genomic DNA was extracted from rat brain and decalcified bone tissue using the DNeasy blood and tissue Mini Kit (Qiagen, Hilden, Germany) with minor modifications in manufacturer's protocol.

For some tissues, such as decalcified bone, this is a particularly problematic issue because the bone in the section is often thicker than the surrounding tissue.

Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage.

The well-oriented decalcified bone sections helped to verify the extent of cement penetration and the immediate interface reaction to the cement.

Following the introduction of 2-chlorodeoxyadenosine (2-CDA) for the treatment of HCL, the role of therapeutic splenectomy has decreased, and after a single cycle of 2-CDA, the tumor burden in the bone marrow may be reduced to remission patterns.[8] To enable evaluation of specimens with a low tumor burden, bone marrow biopsies are stained with L-26, a B-call marker that works well in decalcified bone marrow biopsies.[9] CD3 immunostaining should also be included to assess normal background levels of lymphocytes in biopsy specimens.