This cut the time required in decalcification, minimized the damages caused by the prolonged contact of decalcification solution to the structure of the tooth and also removed the very critical step of setting of the decalcified
tooth in the correct orientation while making of wax block.
The temporal bones were removed, immersed in 70% ethanol/RNase-free water for 6 h at 4[degrees]C, and decalcified
in 0.12 M EDTA including RNA later (Thermo Fisher Scientific, Waltham, MA, USA) for approximately 5 days at 4[degrees]C and then embedded in OCT tissue compound for LCM.
Caption: Figure 5: H and E stained decalcified
section shows the presence of eosinophilic tubular structure resembling dentin surrounding the pulpal space interspersed with dense collagen fibers, endothelial lined blood vessels, and few inflammatory cells chiefly composed of lymphocytes.
The tissues were then decalcified
and prepared for embedding into paraffin blocks.
The cochleae from Myo3a mutant and wild-type mice were removed, fixed with 4% formaldehyde in 10 mM phosphate buffer at 4[degrees]C overnight, decalcified
in 10% EDTA in 10 mM phosphate-buffered saline (PBS) at room temperature for 2 d, dehydrated with 30% to 100% ethanol series, and treated with xylene for transparency.
Once the tumor was decalcified
, two cut surfaces were processed for microscopic examination.
Samples were taken from the mass, fixed in formalin, decalcified
, and sent for further histopathological analysis.
Cr in decalcified
tissues to explore its possible mode of action.
The tissue was immersed in formalin solution for fixation and subsequently decalcified
. The interface between the tumor and normal bone marrow was grossly determined and submitted for microscopic evaluation to detect the relationship between the tumor and bone marrow and identify the transition type and width.
The results were very similar, except non-CAP PT laboratories were much less concerned about validating assays in decalcified
specimens (22.4% [147 of 656] of CAP laboratories versus 7.7% [2 of 26] of non-CAP laboratories) and cytology specimens (20.6% [135 of 656] versus 3.8% [1 of 26]) and were less likely to use the guidelines for validating predictive marker assays (75.6% [500 of 661] versus 50.0% [13 of 26]).
Afterward, the tissues were decalcified
at room temperature in a solution prepared from 0.1 M Na-EDTA (Sigma, Darmstadt, Germany) at pH 7.3 for 2 weeks.
Oesterle  decalcified
thirty temporal bones from embryos up to 66-year-old humans and cut them in 15-25 [micro]m thick slices, but not every section was analyzed.
Bone samples were decalcified
in 10% EDTA (pH 7.2) at 4 [degrees]C for 21 days.
After a first scan, bones are then decalcified
and incubated with a solution of phosphotungstic acid (PTA), which enhances gelatin X-ray contrast by binding to the contained collagens.