Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectra (MS) were acquired on a MALDI-TOF/TOF Autoflex II spectrometer (Bruker Daltonics
, Germany) in the reflector positive ion mode with an acceleration voltage of 20 kV, a delay time of 150 ns and an acquisition mass range of 800 to 3200 Da.
High resolution mass spectrometry was carried out using a MicroTOF QIII Bruker Daltonic
using an ESI positive ionization with the following settings: capillary voltage: 4500 V; nebulizer pressure: 1.2 bar; drying gas: 8 L/min at 200[degrees]C.
Proteins in the spots were in-gel digested, and peptide-mass fingerprints was obtained by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (Bruker Daltonic
, MA, USA).
The chromatographic system was coupled to a time-of-flight (TOF) mass spectrometer (MS) (Bruker Daltonic
Bremen, Germany) that was equipped with an orthogonal electrospray interface (ESI; model G1607A from Agi-lent Technologies, Palo Alto, CA, USA) that operated in negative and positive modes of ionisation.
The Nottingham-based company said sales rose to pounds 7.1 million from pounds 3.8 million boosted by its defence business through a strategic partnership with Bruker Daltonic
, a supplier of substance detection systems.
introduced its Apex IV FT-MS at Pittcon 2002, which is the next evolution in its Apex series of FT mass spectrometers.