Freeze-fracture analysis disclosed the presence of both gap junctions and tight-junction sealing strands in the UROtsa cells cultured
on serum-free growth medium (Figure 3 A,B,C).
In Dixon's notoriously racist novel, the only prominent black dialect speakers die, and George Harris, Jr., the cultured
, Northern-educated African American, is clearly represented as the "standard"-speaking standard bearer for the black race in the twentieth century.
In 1838, members of the Manchester Athenaeum, a middle-class forum for lectures and cultured
leisure, attempted to exclude Unitarians from holding office.
In order to do so, she created a protagonist, Harriet Freeman, who left her cultured
and advantaged home in Minneapolis to stay with her great-grandmother who, although she could not read or write, had a strength and nobility Harriet admired.
Although typically only persons with epidemiologic linkage to index patients are cultured
for possible GAS carriage, the circumstances described below resulted in more widespread collection of culture specimens.