Experiments using black pepper extract suggest that the anti-secretory, anti-motility and anti-diarrheal activities possessed by crude extract
might be due to the presence of carbohydrates and alkaloids.
Cytotoxic brine shrimp assay: Stock solution (5mg/ml) of methanol crude extract
of Erythraea ramosissima was prepared in methanol for this cytotoxic brine shrimp assay.
serpens Wall crude extract
and the subsequent fractions, n-hexane, chloroform, ethyl acetate and aqueous at different doses 1000 and 2000 mg/kg, i.
asperellum at 500 [micro]l/ml crude extract
concentration (TA 500) significantly obtained the highest percentage with a mean value of 24.
Similar study conducted also showed that cacao pulp crude extract
was inactive against P aeruginosa ATCC 27853 (Panganiban et al.
Before blending, semifinished products were examined for the following parameters: alcohol according to GOST R 51135-2010, mass concentration of crude extract
with refractometric method according to GOST R 51135-2010, organoleptic indicators according to GOST R 52522-2006.
In pure hexane solvent, yellow colored bands move equally from crude extract
of all basil genotypes on TLC plate (Fig.
With distilled water Electrophoresis in polyacrylamide gel for the crude extract
using distilled water yield a variety of results, supporting the results found in several studies.
Briefly, the crude extracts
(10 g of AML and AL each) were dispersed separately into 100 mL of double distilled water, and the solutions were partitioned with 100 mL of n-hexane by stirring for 60 min at room temperature to remove any residual lipids.
Two normal cell lines were also used to investigate the cytotoxicity of the crude extract
Human bronchial epithelial cells (BEAS-2B) were cultured in bronchial epithelial cell growth medium (BEGM) and the mouse fibroblast cells (3T3) were cultured in DMEM with all the supplements mentioned above.
50] estimated for the crude extract
, and the same experimental procedures described previously for the screening assay were adopted.
Thus, our objective was to compare the effect of crude extract
from a strain of microcystin-producing M.
The resulting crude extract
was suspended in water and extracted with solvents of different polarities.
An aliquot of crude extract
was spotted onto the silica gel plate and allowed to dry for two minutes.
2005) reported the larvicidal activity of the methanol crude extract