The mean sperm concentrations per Neubauer counting chamber
were >1 x [10.sup.6]/mL with progressive motility of >30% in all three estimated categories, indicating that these samples could theoretically be suitable for three to four IUI cycles.
where [C.sub.1] is the total oocyst count on the left side of the counting chamber
, [C.sub.2] is the total oocyst count on the right side of the counting chamber
, 0.3 is the total volume in ml from each side of the McMaster counting chamber
, 10 is the total ml of the dilution factor, Y is the total volume in ml of fecal- [K.sub.2][Cr.sub.2][O.sub.7] suspension, and 7 is the number of mice in each dietary group.
Some of its other features include a disposable counting chamber
, trypan blue viability, providing live images, estimating cell size, and saving date and cell images.
In addition, stained samples may be read on a Hausser counting chamber
at 40x magnification instead of 100x magnification because the natural cells enlarge more during RFTM incubation than in vitro cultured cells, stain characteristically and are easily observed under 40x magnification.
Pyuria: deposit in high-power microscopic field--WBC/ hpf--versus WBC/mm3 in counting chambers
. Reappraisal of a valuable clinical routine method (urinary sediment).
A sterile swab is moistened with a wetting agent; an area of interest is swabbed; the swab is mixed with a nucleotide releasing agent in a cuvette; a luciferin-luciferase reagent is added, and the light emitted is read in a counting chamber
. The relative light units provide an indication of the ATP present on the swabbed surface.
Platelets were counted using improved Neubauer ruled counting chamber
(Hawksley, UK) and the number of platelets per litre of blood was calculated using the first principle.
If the concentration of the beads is too high then the cells will overlap on the counting chamber
and are difficult to count.
Alternatively, manual chamber counting methods, which have been around for many years, can be used but come with a number of potential inherent sources of error (dilutional errors, dirty or broken pipettes, counting chamber
imperfections, and chamber filling errors).
Generally, good agreement was obtained between RBC and WBC counts by the UF-100 and the counting chamber
. Although no major discrepancies were observed, UF-100 counts, especially the high RBC and WBC counts, were lower than the microscopic counts.
[18,19] Alternative methods include enumeration of leukocytes using a counting chamber
, which has been found to correlate well with the leukocyte excretion rate.
The WBC's were stained with WBC diluting fluid (Turks-water: glacial acetic acid: gentian violet =97:2:1) followed by counting of cells in the haemocytometer using 400x magnification after filling the counting chamber
by means of a glass capillary (Fluid depth at 0.1 mm).
It was determined that, because the targets were so low, any signal generated by a dirty flow cell, dirty optical, or dirty counting chamber
would appear in the monocyte count and weigh in on the results, thus producing a proficiency failure.