counterstain


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Words related to counterstain

a stain of contrasting color that is used when the principal stain does not show the structure clearly

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Sodium-potassium ATPase (red signal) was used as a counterstain for the cells' basolateral membranes.
DAPI was used as the nucleus counterstain. Scale bar: 50 [micro]m.
Alexa Fluor 555 (red) and Alexa Fluor 488 (green) and with Hoechst 33342 nuclear counterstain (blue).
Immunoperoxidase with hematoxylin counterstain, bar = 200 [micro]m.
Immunohistochemical reaction (IHC) with 3,3'-diaminobenzidine (DAB) chromogen and hematoxylin counterstain. Bar = 60 [micro]m (C) and 80 [micro]m (D).
(c, d) Transient receptor potential vanilloid 4 in synovial tissues of normal and knee osteoarthritis groups detected by indirect immunofluorescence (immunohistochemistry, 3, 3'-diaminobenzidine (DAB) as organic reagent, hematoxylin counterstain, x400).
Staining was finally visualized using 3,3-diaminobenzidine (DAB; Vector laboratories) as chromogen, and counterstain was performed with a diluted hematoxylin solution for 1 min.
Cells were collected by centrifugation at 500g for 5 min, and Annexin V-FITC reagent (BD Bioscience) with or without PI counterstain was used to resuspend the pellet.
Visualization was performed using the Leica Refine kit with DAB chromogen and hematoxylin counterstain. Slides were dehydrated, cleared, and mounted with Tissue-Tek Glass Mounting Medium (Sakura).
DSBs (double stands breaks) were labeled by FITC-dUTP for staining and propidium iodine (PI) as a counterstain (APO-DIRECT Kit, BD).
Caption: Figure 2: (a) High power view shows the tumour cells staining positive for vimentin (IH stain with haematoxylin counterstain x400 magnification).
Immunoperoxidase stain with antibody against OCT 3/4 shows absence of germ cell neoplasia in situ in atrophic tubules (e) (DAB and hematoxylin counterstain, x200).
We used 3,39-diaminobenzidine for visualization as well as hematoxylin as a counterstain. According to the Fromowitz score system, we randomly selected six high magnification fields (x400) in each kidney section.
Zingiber officinale extract solution stained the muscle fibers and cytoplasm yellow and the nuclei deep green within 4 min when it was used as a counterstain for hematoxylin.