Effect of pH and sucrose concentration on conidium
size and septation in four Bipolaris species.
After 24 h a single germinated conidium
was spotted by a microscope a portion of the media representing merely marked conidium
was excised and shifted to PDA and these plates were retained at 25C.
The conidial chain with isthimi consists of seven conidia and aggregates in slimy masses, single conidium
smooth, gutullate, aseptate, and the conidia remain attached to one another by narrow isthimi (Figures 3 and 4).
There was no apparent prominence of either the conidium
shape or the presence of fibrosin bodies associated with any specific hybrid.
A minimum of400 conidia were examined for germination; a conidium
was considered viable (germinated) if it had produced a visible germination peg during the specified incubation time.
The loop with the accumulated conidia was streaked across a dish of WA and incubated for 12 to 30 hours at 28 [degrees]C after which a single germinating conidium
was transferred to an SNA plate for inoculum production.
Figure lA shows a mature conidium
and figure lB shows a chlamydospore.