3b), with conidia, smooth-walled and olivaceous green conidia present on Conidiophore
with broom like appearance (Fig.
The hyphae are brown, septate, and smooth produced branched or unbranched conidiophore
, lateraly or on the apex.
septate and brown in color, occasionally producing a###Bolus###4
6 [micro]m, borne apically on a short conidiophore
arising from PC (Fig.
Morphological Key Morphological Observation Species Character Colony growth rate (cm) 7-8 cm Colony colour Dark yellowish green Reverse colony colour Colourless Colony edge Smooth Culture smell No characteristic odour Mycelial form Smooth Mycelial colour Cottony white Cultural characteristic Cultural Character Species Character Conidiation Ring like zones Conidiophore
branching Branched, regular Phialide shape Cyllindrical Phialide size 6.
8 A conidiophore
carries a distinctive sterigmata characteristic of this fungus.
All the isolates were identified up to species level based on phenotypic characters like colony colour and growth; size and shape of conidiophore
, phialides and conidia.
Isolate was identified up to species level based on phenotypic characters like colony colour and growth; size and shape of conidiophore
, phialides and conidia using the available literature (Bisset 1991a,1991b and Samuels et al.
Alternaria###6-7 cm###dark brown###Hyphae branched and conidiophore
simple,###conidia formed in long
Microscopic features observed were radiate conidia, thin walled hyaline vesicle, metulae covered entire vesicle, brownish conidiophore
and pink to venaceous purple sclerotia.
branching: Highly branched, regular
Monoconidial (genetically pure single spore culture) isolate of Tr(CSAU)/7284 was used (Table 1), which was identified by slide mounting and microscopic observation based on phenotypic characters like colony colour and growth, size and shape of conidiophore
, phialides and conidia, using the available literature (9,10,11) that was later confirmed by Indian Type Culture Collection (ITCC), Division of Plant Pathology IARI, New Delhi-12.
spirale Floccose Cottony Ring like to white arachnoid Name of Conidiophore
Conidial Conidial Chlamydo- Strains branching wall colour spores T.
a) colony morphology on PDA, (b) conidia in chains, (c) chains of conidia on strigmata (low magnification), (d) conidia (high magnification), (e-i) conidiophore
and spear shaped strigmata.
In anamorph, pear-shaped pycnidia contain unicellular or bicellular pycnidiospores (6-23 um) produced upon conidiophores
(Punithalingam and Holliday, 1972; Nene, 1982).