To conclude, while pyrene units at the tandem junction are likely to cause destabilization of corresponding duplexes with complementary DNA
target, an attendant effect of stabilization caused by hydrophobic interactions between multiple pyrene units at the junction resulted in difference of only 2.1[degrees]C between [T.sub.m] values of duplexes (IVb+IIb) x fcDNA and (IV+II) x fcDNA.
In a statement issued after the decision was announced, the company noted that the Court "upheld its patents on complementary DNA
This can also integrate the HERV RNA complementary DNA
into the noncoding region of eukaryotic non coding DNA using HERV integrase .
However, nobody had succeeded in cloning a full-length [alpha]-globin complementary DNA
(cDNA) copy of the mRNA.
The researchers assembled snippets of genetic code from healthy human prostate tissue into a complementary DNA
In the present study, we included complementary DNA
nucleotide sequences for each gene.
As DNA microarrays have evolved from in-house complementary DNA
arrays to robust commercial oligonucleotide platforms, there has been a migration to higher density biochips with increasing content and better analytical methodologies.
Because they tagged the complementary DNA
with three differently colored fluorescent dyes, the attached DNA showed that the wires were in the proper places.
Thus, the enzyme requires a single strand for a template and a small RNA fragment for the synthesis of a complementary DNA
As a result of morphological examination and complementary DNA
analyses, these 5 species are considered synonyms of S.
This homogeneous phase detection assay is based on the simultaneous binding of 2 aptamer-containing oligonucleotides, each linked to a complementary DNA
reporter sequence containing 2 reactive fluorophore precursor molecules.
RNA was extracted from 250 [micro]L virus-infected culture supernatant by using Trizol LS (Invitrogen, Carlsbad, CA, USA), and the complementary DNA
was synthesized with Oligo(dT)15 (Invitrogen) by using SuperScriptII reverse transcriptase (Invitrogen).
Under normal circumstances, nucleic acid sequences that have large stretches of G residues have a tendency to bind together instead of binding to their complementary DNA
strand, making those sequences extremely difficult to design assays to detect.
mRNA are converted into complementary DNA
(cDNA) and labeled red (infected) and green (noninfected).
At the end of the treatment periods, mRNA was extracted from the cells, pooled according to treaunent and cell line, and used to create labeled complementary DNA